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验证数据展示

  • WB analysis of mouse testis using 12670-1-AP

    WB analysis of mouse testis using 12670-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12670-1-AP (AGFG1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 12670-1-AP

    IHC staining of human breast cancer using 12670-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 12670-1-AP (AGFG1 antibody) at dilution of 1:100 (under 10x lens).

  • IP experiment of mouse testis using 12670-1-AP

    IP experiment of mouse testis using 12670-1-AP

    IP result of anti-AGFG1 (IP:12670-1-AP, 4ug; Detection:12670-1-AP 1:400) with mouse testis tissue lysate 4000ug.

AGFG1 Polyclonal antibody

AGFG1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 12670-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, IHC, ELISA and More (1)

别名

AGFG1, ArfGAP with FG repeats 1, HIV 1 Rev binding protein, HRB, Nucleoporin like protein RIP, RAB, Rev interacting protein, RIP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, HeLa cells, HepG2 cells, HT-1080 cells, Jurkat cells, K-562 cells
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12670-1-AP targets AGFG1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性mouse
免疫原 AGFG1 fusion protein Ag3352 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ArfGAP with FG repeats 1
别名 AGFG1, ArfGAP with FG repeats 1, HIV 1 Rev binding protein, HRB, Nucleoporin like protein RIP, RAB, Rev interacting protein, RIP
计算分子量 562 aa, 58 kDa
观测分子量 58 kDa
GenBank蛋白编号BC030592
基因名称 AGFG1
Gene ID (NCBI) 3267
RRIDAB_2258077
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP52594
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for AGFG1 antibody 12670-1-APDownload protocol
IHC protocol for AGFG1 antibody 12670-1-APDownload protocol
IP protocol for AGFG1 antibody 12670-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Cell

A Translation-Activating Function of MIWI/piRNA during Mouse Spermiogenesis.

Authors - Peng Dai
View Article

Proc Natl Acad Sci U S A

Biogenesis of sperm acrosome is regulated by pre-mRNA alternative splicing of Acrbp in the mouse.

Authors - Yoshinori Kanemori
View Article