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验证数据展示

  • WB analysis using 32053-1-AP

    WB analysis using 32053-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32053-1-AP (AGER/RAGE antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 32053-1-AP

    IHC staining of mouse lung using 32053-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 32053-1-AP (AGER/RAGE antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat lung using 32053-1-AP

    IHC staining of rat lung using 32053-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 32053-1-AP (AGER/RAGE antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

AGER/RAGE Polyclonal antibody

AGER/RAGE Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32053-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, ELISA

别名

AGER, RAGE, Advanced glycosylation end product-specific receptor

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse lung tissue, rat lung tissue
Positive IHC detected inrat lung tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32053-1-AP targets AGER/RAGE in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse, rat
免疫原 AGER/RAGE fusion protein Eg1135 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 advanced glycosylation end product-specific receptor
别名 AGER, RAGE, Advanced glycosylation end product-specific receptor
计算分子量43 kDa
观测分子量42-55 kDa
基因名称 Ager
Gene ID (NCBI) 81722
RRIDAB_3670178
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ63495
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Advanced glycosylation end product-specific receptor (AGER, also known as RAGE) is a member of the immunoglobulin superfamily of cell surface receptors, which interacts with distinct families of ligands, mediating diverse functions in a broad array of cell types including cellular migration, proliferation, survival and apoptosis (PMID: 12645002; 17425919). It senses endogenous stress signals with a broad ligand repertoire including advanced glycation end products, S100 proteins, high-mobility group box 1 protein/HMGB1, amyloid beta/APP oligomers, nucleic acids, phospholipids and glycosaminoglycans (PMID: 19910580; 28627626). It interacts with distinct molecules implicated in homeostasis, development, inflammation, and certain diseases such as diabetes and Alzheimer's disease (PMID: 26253613; 31079281).

实验方案

Product Specific Protocols
WB protocol for AGER/RAGE antibody 32053-1-APDownload protocol
IHC protocol for AGER/RAGE antibody 32053-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols