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验证数据展示

  • WB analysis of pig lung using 66833-1-Ig (same clone as 66833-1-PBS)

    WB analysis of pig lung using 66833-1-Ig (same clone as 66833-1-PBS)

    pig lung tissue were subjected to SDS PAGE followed by western blot with 66833-1-Ig (AGER antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66833-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung using 66833-1-Ig (same clone as 66833-1-PBS)

    IHC staining of human lung using 66833-1-Ig (same clone as 66833-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66833-1-Ig (AGER antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66833-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AGER/RAGE antibody included
  • IHC staining of human lung using 66833-1-Ig (same clone as 66833-1-PBS)

    IHC staining of human lung using 66833-1-Ig (same clone as 66833-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66833-1-Ig (AGER antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66833-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AGER/RAGE antibody included
  • IF Staining of mouse lung using 66833-1-Ig (same clone as 66833-1-PBS)

    IF Staining of mouse lung using 66833-1-Ig (same clone as 66833-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using AGER/RAGE antibody (66833-1-Ig, Clone: 3D4G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66833-1-PBS in a different storage buffer formulation.

  • IF Staining of rat lung using 66833-1-Ig (same clone as 66833-1-PBS)

    IF Staining of rat lung using 66833-1-Ig (same clone as 66833-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat lung tissue using AGER antibody (66833-1-Ig, Clone: 3D4G11 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66833-1-PBS in a different storage buffer formulation.

AGER/RAGE Monoclonal antibody, PBS Only

AGER/RAGE Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66833-1-PBS

产品说明书

CloneNo. 3D4G11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF-P, ELISA

别名

AGER, RAGE, 3D4G11, Advanced glycosylation end product-specific receptor

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy AGER/RAGE Ready-To-Use IHC Kit. AGER/RAGE primary antibody included.

产品信息

66833-1-PBS targets AGER/RAGE in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag9657

Product name: Recombinant human AGER protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 26-340 aa of BC020669

Sequence: ITARIGEPLVLKCKGAPKKPPQRLEWKLNTGRTEAWKVLSPQGGGPWDSVARVLPNGSLFLPAVGIQDEGIFRCQAMNRNGKETKSNYRVRVYQIPGKPEIVDSASELTAGVPNKVGTCVSEGSYPAGTLSWHLDGKPLVPNEKGVSVKEQTRRHPETGLFTLQSELMVTPARGGDPRPTFSCSFSPGLPRHRALRTAPIQPRVWEPVPLEEVQLVVEPEGGAVAPGGTVTLTCEVPAQPSPQIHWMKDGVPLPLPPSPVLILPEIGPQDQGTYSCVATHSSHGPQESRAVSISIIEPGEEGPTAGSVGGSGLGT

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 advanced glycosylation end product-specific receptor
别名 AGER, RAGE, 3D4G11, Advanced glycosylation end product-specific receptor
计算分子量 404 aa, 43 kDa
观测分子量 45 kDa
GenBank蛋白编号BC020669
基因名称 AGER
Gene ID (NCBI) 177
RRIDAB_2882176
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ15109
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Advanced glycosylation end product-specific receptor (AGER, also known as RAGE) is a multiligand receptor of the immunoglobulin superfamily. It interacts with distinct molecules implicated in homeostasis, development, and inflammation, and certain diseases such as diabetes and Alzheimer disease.

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