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AGBL5/CCP5 Polyclonal antibody

AGBL5/CCP5 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 15569-1-AP

Print datasheet

Synonyms

AGBL5, AGBL5/CCP5, ATP/GTP binding protein like 5, CCP5

验证数据展示

  • WB analysis of recombinant protein using 15569-1-AP

    WB analysis of recombinant protein using 15569-1-AP

    WB result of 15569-1-AP (anti-CCP5): Fusion protein expressed in Baculovirus.(From Dr. Jason Ford).

  • IHC staining of mouse cerebellum using 15569-1-AP

    IHC staining of mouse cerebellum using 15569-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15569-1-AP (AGBL5/CCP5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 15569-1-AP

    IHC staining of mouse cerebellum using 15569-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15569-1-AP (AGBL5/CCP5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U-251 using 15569-1-AP

    IF Staining of U-251 using 15569-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using AGBL5/CCP5 antibody (15569-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inrecombinant protein
Positive IHC detected inmouse cerebellum tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU-251 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15569-1-AP targets AGBL5/CCP5 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF, IHC, WB, ELISA
Tested Reactivity human, mouse
Immunogen AGBL5/CCP5 fusion protein Ag7889 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATP/GTP binding protein-like 5
Synonyms AGBL5, AGBL5/CCP5, ATP/GTP binding protein like 5, CCP5
Calculated Molecular Weight98 kDa
GenBank Accession NumberBC007415
Gene Symbol AGBL5
Gene ID (NCBI) 60509
RRIDAB_2878151
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NDL9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for AGBL5/CCP5 antibody 15569-1-APDownload protocol
IF protocol for AGBL5/CCP5 antibody 15569-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols