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验证数据展示

  • WB analysis of mouse liver using 16990-1-AP

    WB analysis of mouse liver using 16990-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16990-1-AP (AGBL3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 16990-1-AP

    IHC staining of human lung cancer using 16990-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16990-1-AP (AGBL3 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lung cancer using 16990-1-AP

    IHC staining of human lung cancer using 16990-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16990-1-AP (AGBL3 Antibody) at dilution of 1:50 (under 40x lens).

  • Featured Product
  • KD/KO验证

AGBL3 Polyclonal antibody

AGBL3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16990-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

AGBL3, ATP/GTP binding protein like 3, CBPC3, CCP3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue, L02 cells
Positive IHC detected inhuman lung cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16990-1-AP targets AGBL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性mouse
免疫原 AGBL3 fusion protein Ag10592 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATP/GTP binding protein-like 3
别名 AGBL3, ATP/GTP binding protein like 3, CBPC3, CCP3
计算分子量 73 kDa, 116 kDa
观测分子量 73 kDa
GenBank蛋白编号BC030651
基因名称 AGBL3
Gene ID (NCBI) 340351
RRIDAB_2878337
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NEM8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AGBL3, also named as CCP3, is a metallocarboxypeptidase that may play a role in the processing of tubulin. AGBL3 has some isoforms with MW 107 kDA, 73 kDa, 116 kDa and 20 kDa.

实验方案

Product Specific Protocols
WB protocol for AGBL3 antibody 16990-1-APDownload protocol
IHC protocol for AGBL3 antibody 16990-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Exp Med

Glutamylation of deubiquitinase BAP1 controls self-renewal of hematopoietic stem cells and hematopoiesis.

Authors - Zhen Xiong
  • KO Validated
View Article