验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, L02 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27681-1-AP targets AGAP3 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag26662 Product name: Recombinant human AGAP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 474-556 aa of BC044644 Sequence: LAIGPCKSLPNSPSHSAVSAASIPAVHINQATNGGGSAFSDYSSSVPSTPSISQRELRIETIAASSTPTPIRKQSKRRSNIFT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ArfGAP with GTPase domain, ankyrin repeat and PH domain 3 |
| 别名 | AGAP-3, Centaurin gamma 3, CENTG3, CRAG, MRIP-1 |
| 计算分子量 | 95 kDa |
| 观测分子量 | 95 kDa |
| GenBank蛋白编号 | BC044644 |
| 基因名称 | AGAP3 |
| Gene ID (NCBI) | 116988 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96P47 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AGAP3, also known as MRIP-1, CENTG3, contains multiple signaling domains, a GTPase-like domain, a pleckstrin homology domain, and an ArfGAP domain, and exists as a component of the NMDA receptor complex. AGAP3 is a component of the NMDA receptor complex that regulates Arf6 and Ras/ERK signaling (PMID: 23904596).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AGAP3 antibody 27681-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
