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验证数据展示

  • IF Staining of HepG2 using CL488-82982-5

    IF Staining of HepG2 using CL488-82982-5

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 AFP antibody (CL488-82982-5, Clone: 230435A2 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated AFP Recombinant antibody

AFP Recombinant Antibody for IF/ICC
Cat No. CL488-82982-5

产品说明书

CloneNo. 230435A2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

230435A2, Alpha fetoglobulin, alpha fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy AFP Ready-To-Use IHC Kit. AFP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 82982-5-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82982-5 targets AFP in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 AFP fusion protein Eg0539 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 alpha-fetoprotein
别名 230435A2, Alpha fetoglobulin, alpha fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin
计算分子量 69 kDa
观测分子量68-72 kDa
GenBank蛋白编号BC027881
基因名称 AFP
Gene ID (NCBI) 174
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP02771
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AFP (Alpha-fetoprotein) is a major plasma protein in the fetus and its concentration is very low in the adult (PMID:24120489). AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma, indicating that AFP can serve as a tumor marker (PMID: 18669658). AFP is also a glycosylated protein and based on its binding capability to lectin Lens Culinaris Agglutinin (LCA), and total AFP can be separated into three different glycoforms, AFP-L1, AFP-L2, and AFP-L3. Core-fucosylated form of AFP (AFP-L3) is a more specific indicator than total AFP for HCC (PMID: 33128033, 35458505)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 AFP antibody CL488-82982-5Download protocol
Standard Protocols
Click here to view our Standard Protocols