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验证数据展示

  • WB analysis of HepG2 using 67852-1-Ig (same clone as 67852-1-PBS)

    WB analysis of HepG2 using 67852-1-Ig (same clone as 67852-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67852-1-Ig (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67852-1-PBS in a different storage buffer formulation.

  • WB analysis of HuH-7 using 67852-1-Ig (same clone as 67852-1-PBS)

    WB analysis of HuH-7 using 67852-1-Ig (same clone as 67852-1-PBS)

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 67852-1-Ig (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67852-1-PBS in a different storage buffer formulation.

  • WB analysis of human testis using 67852-1-Ig (same clone as 67852-1-PBS)

    WB analysis of human testis using 67852-1-Ig (same clone as 67852-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 67852-1-Ig (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67852-1-PBS in a different storage buffer formulation.

  • WB analysis of human placenta using 67852-1-Ig (same clone as 67852-1-PBS)

    WB analysis of human placenta using 67852-1-Ig (same clone as 67852-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67852-1-Ig (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67852-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67852-1-Ig (same clone as 67852-1-PBS)

    IHC staining of human liver cancer using 67852-1-Ig (same clone as 67852-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67852-1-Ig (AFP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67852-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AFP antibody included
  • IHC staining of human liver cancer using 67852-1-Ig (same clone as 67852-1-PBS)

    IHC staining of human liver cancer using 67852-1-Ig (same clone as 67852-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67852-1-Ig (AFP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67852-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AFP antibody included
  • IHC staining of human liver using 67852-1-Ig (same clone as 67852-1-PBS)

    IHC staining of human liver using 67852-1-Ig (same clone as 67852-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67852-1-Ig (AFP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67852-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AFP antibody included
  • IHC staining of human liver using 67852-1-Ig (same clone as 67852-1-PBS)

    IHC staining of human liver using 67852-1-Ig (same clone as 67852-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67852-1-Ig (AFP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67852-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AFP antibody included

AFP Monoclonal antibody, PBS Only

AFP Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67852-1-PBS

产品说明书

CloneNo. 4B3E4

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

4B3E4, Alpha fetoglobulin, alpha fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy AFP Ready-To-Use IHC Kit. AFP primary antibody included.

产品信息

67852-1-PBS targets AFP in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag6089

Product name: Recombinant human AFP protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 260-609 aa of BC027881

Sequence: LDVAHVHEHCCRGDVLDCLQDGEKIMSYICSQQDTLSNKITECCKLTTLERGQCIIHAENDEKPEGLSPNLNRFLGDRDFNQFSSGEKNIFLASFVHEYSRRHPQLAVSVILRVAKGYQELLEKCFQTENPLECQDKGEEELQKYIQESQALAKRSCGLFQKLGEYYLQNAFLVAYTKKAPQLTSSELMAITRKMAATAATCCQLSEDKLLACGEGAADIIIGHLCIRHEMTPVNPGVGQCCTSSYANRRPCFSSLVVDETYVPPAFSDDKFIFHKDLCQAQGVALQTMKQEFLINLVKQKPQITEEQLEAVIADFSGLLEKCCQGQEQEVCFAEEGQKLISKTRAALGV

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 alpha-fetoprotein
别名 4B3E4, Alpha fetoglobulin, alpha fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin
计算分子量 69 kDa
观测分子量 68-72 kDa
GenBank蛋白编号BC027881
基因名称 AFP
Gene ID (NCBI) 174
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP02771
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AFP (Alpha-fetoprotein) is a major plasma protein in the fetus and its concentration is very low in the adult (PMID:24120489). AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma, indicating that AFP can serve as a tumor marker (PMID: 18669658). AFP is also a glycosylated protein and based on its binding capability to lectin Lens Culinaris Agglutinin (LCA), and total AFP can be separated into three different glycoforms, AFP-L1, AFP-L2, and AFP-L3. Core-fucosylated form of AFP (AFP-L3) is a more specific indicator than total AFP for HCC (PMID: 33128033, 35458505)