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AFM Polyclonal antibody

AFM Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, WB,ELISA

Conjugate

Unconjugated

Cat No : 19494-1-AP

Print datasheet

Synonyms

afamin, AFM, ALB2, ALBA, ALF, Alpha Alb, Alpha albumin

验证数据展示

  • WB analysis of human plasma using 19494-1-AP

    WB analysis of human plasma using 19494-1-AP

    human blood were subjected to SDS PAGE followed by western blot with 19494-1-AP (AFM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 19494-1-AP

    IF Staining of HepG2 using 19494-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 19494-1-AP (AFM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman blood tissue
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

19494-1-AP targets AFM in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name afamin
Synonyms afamin, AFM, ALB2, ALBA, ALF, Alpha Alb, Alpha albumin
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberNM_001133
Gene Symbol AFM
Gene ID (NCBI) 173
RRIDAB_2878581
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43652
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AFM (Afamin) is a member of the albumin superfamily, which comprises albumin, vitamin D-binding protein, a-fetoprotein, and afamin. AFM is present in the plasma/serum, cerebrospinal fluid, and follicular fluid, and functions as a vitamin E-binding protein. Alterations of expression level of AFM have been linked to variety of diseases like cancer.

实验方案

Product Specific Protocols
WB protocol for AFM antibody 19494-1-APDownload protocol
IF protocol for AFM antibody 19494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell Endocrinol

Afamin promotes glucose metabolism in papillary thyroid carcinoma.

Authors - Chen-Tian Shen
View Article