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验证数据展示

  • WB analysis using 68517-1-Ig

    WB analysis using 68517-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68517-1-Ig (AFG3L2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 68517-1-Ig

    IF Staining of MCF-7 using 68517-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using AFG3L2 antibody (68517-1-Ig, Clone: 1C12F3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

  • FC experiment of HeLa using 68517-1-Ig

    FC experiment of HeLa using 68517-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug AFG3L2 Monoclonal antibody (68517-1-Ig, Clone:1C12F3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

AFG3L2 Monoclonal antibody

AFG3L2 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 68517-1-Ig

产品说明书

CloneNo. 1C12F3

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IF/ICC, FC (Intra), ELISA

别名

1C12F3, AFG3 like protein 2, AFG3L 2, AFG3-like protein 2, EC:3.4.24.-

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, LNCaP cells, HepG2 cells, Jurkat cells, K-562 cells, pig heart tissue, rabbit heart tissue, rat heart tissue, mouse heart tissue
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68517-1-Ig targets AFG3L2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag7180

Product name: Recombinant human AFG3L2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 449-797 aa of BC065016

Sequence: ILAGTNRPDILDPALLRPGRFDRQIFIGPPDIKGRASIFKVHLRPLKLDSTLEKDKLARKLASLTPGFSGADVANVCNEAALIAARHLSDSINQKHFEQAIERVIGGLEKKTQVLQPEEKKTVAYHEAGHAVAGWYLEHADPLLKVSIIPRGKGLGYAQYLPKEQYLYTKEQLLDRMCMTLGGRVSEEIFFGRITTGAQDDLRKVTQSAYAQIVQFGMNEKVGQISFDLPRQGDMVLEKPYSEATARLIDDEVRILINDAYKRTVALLTEKKADVEKVALLLLEKEVLDKNDMVELLGPRPFAEKSTYEEFVEGTGSLDEDTSLPEGLKDWNKEREKEKEEPPGEKVAN

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 AFG3 ATPase family gene 3-like 2 (yeast)
别名 1C12F3, AFG3 like protein 2, AFG3L 2, AFG3-like protein 2, EC:3.4.24.-
计算分子量 88 kDa
观测分子量80 kDa
GenBank蛋白编号BC065016
基因名称 AFG3L2
Gene ID (NCBI) 10939
RRIDAB_3085226
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y4W6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AFG3L2 is the catalytic subunit of the m-AAA protease, an ATP-dependent proteolytic complex of the mitochondrial inner membrane that degrades misfolded proteins and regulates ribosome assembly(PMID:17101804). Human AFG3L2 is an 80-kDa protein encoded by a 17-exon gene and highly and selectively expressed in human cerebellar Purkinje cells(PMID:20208537) and it can exsit as a truncated 65 kDa protein(PMID:18337413). Defects in AFG3L2 are the cause of spinocerebellar ataxia type 28 (SCA28) and spastic ataxia autosomal recessive type 5 (SPAX5).

实验方案

Product Specific Protocols
FC protocol for AFG3L2 antibody 68517-1-IgDownload protocol
IF protocol for AFG3L2 antibody 68517-1-IgDownload protocol
WB protocol for AFG3L2 antibody 68517-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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