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AFF4 Polyclonal antibody

AFF4 Polyclonal Antibody for WB, IP, ELISA
Cat No. 14662-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA and More (2)

AF4/FMR2 family member 4, AF4/FMR2 family, member 4, AF5Q31, AFF4, MCEF, Protein AF 5q31

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy AFF4 Ready-To-Use IHC Kit. AFF4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14662-1-AP targets AFF4 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, IHC, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 AFF4 fusion protein Ag6336 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 AF4/FMR2 family, member 4
别名 AF4/FMR2 family member 4, AF4/FMR2 family, member 4, AF5Q31, AFF4, MCEF, Protein AF 5q31
计算分子量 127 kDa
观测分子量 140-150 kDa
GenBank蛋白编号BC063007
基因名称 AFF4
Gene ID (NCBI) 27125
RRIDAB_2242609
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UHB7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transcriptional controled by RNA polymerase II (Pol II) is a multi-step process requiring the concerted action of multiple factors and contacts with the DNA template for the proper synthesis of nascent RNA [PMID:12676794]. ELL1 was demonstrated to be a Pol II elongation factor capable of enhancing the catalytic rate of transcription elongation by reducing transient pausing by the enzyme [PMID:8596958]. AFF4 was initially identified as a fusion partner of the MLL (mixed-lineage leukemia) gene participated in infant acute lymphoblastic leukemia, and a component of the ELL/p-TEFb elongation complex. AFF4 interacts with positive transcription elongation factor-b while also repressing Tat transactivation of HIV-1 [PMID:17389929].

实验方案

Product Specific Protocols
WB protocol for AFF4 antibody 14662-1-APDownload protocol
IP protocol for AFF4 antibody 14662-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

Targeting Processive Transcription Elongation via SEC Disruption for MYC-Induced Cancer Therapy.

Authors - Kaiwei Liang
mouseWB

Nat Commun

Transcriptional repression by a secondary DNA binding surface of DNA topoisomerase I safeguards against hypertranscription

Authors - Mei Sheng Lau
human,mouseWB

Mol Cell

RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits

Authors - Yuanjun Li
humanWB

Genes Dev

Acute perturbation strategies in interrogating RNA polymerase II elongation factor function in gene expression.

Authors - Bin Zheng
humanWB

Nucleic Acids Res

Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II.

Authors - Xiaodong Lu
  • KD Validated
humanWB

Cell Discov

Structural and functional insight into the effect of AFF4 dimerization on activation of HIV-1 proviral transcription

Authors - Dan Tang
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