验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue |
Positive IP detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14662-1-AP targets AFF4 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IP, ELISA Application Description |
文献引用应用 | WB, IHC, IP, CoIP |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse |
免疫原 | AFF4 fusion protein Ag6336 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | AF4/FMR2 family, member 4 |
别名 | AF4/FMR2 family member 4, AF4/FMR2 family, member 4, AF5Q31, AFF4, MCEF, Protein AF 5q31 |
计算分子量 | 127 kDa |
观测分子量 | 140-150 kDa |
GenBank蛋白编号 | BC063007 |
基因名称 | AFF4 |
Gene ID (NCBI) | 27125 |
RRID | AB_2242609 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9UHB7 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Transcriptional controled by RNA polymerase II (Pol II) is a multi-step process requiring the concerted action of multiple factors and contacts with the DNA template for the proper synthesis of nascent RNA [PMID:12676794]. ELL1 was demonstrated to be a Pol II elongation factor capable of enhancing the catalytic rate of transcription elongation by reducing transient pausing by the enzyme [PMID:8596958]. AFF4 was initially identified as a fusion partner of the MLL (mixed-lineage leukemia) gene participated in infant acute lymphoblastic leukemia, and a component of the ELL/p-TEFb elongation complex. AFF4 interacts with positive transcription elongation factor-b while also repressing Tat transactivation of HIV-1 [PMID:17389929].
实验方案
Product Specific Protocols | |
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WB protocol for AFF4 antibody 14662-1-AP | Download protocol |
IP protocol for AFF4 antibody 14662-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Targeting Processive Transcription Elongation via SEC Disruption for MYC-Induced Cancer Therapy. | ||
Nat Commun Transcriptional repression by a secondary DNA binding surface of DNA topoisomerase I safeguards against hypertranscription | ||
Mol Cell RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits | ||
Genes Dev Acute perturbation strategies in interrogating RNA polymerase II elongation factor function in gene expression. | ||
Nucleic Acids Res Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II.
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Cell Discov Structural and functional insight into the effect of AFF4 dimerization on activation of HIV-1 proviral transcription |