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验证数据展示

  • WB analysis using 25414-1-AP

    WB analysis using 25414-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25414-1-AP (AFF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of SH-SY5Y using 25414-1-AP

    IP experiment of SH-SY5Y using 25414-1-AP

    IP result of anti-AFF2 (IP:25414-1-AP, 4ug; Detection:25414-1-AP 1:600) with SH-SY5Y cells lysate 1720 ug.

AFF2 Polyclonal antibody

AFF2 Polyclonal Antibody for WB, IP, ELISA
Cat No. 25414-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

AF4/FMR2 family member 2, FMR2, FMR2P, FRAXE, MRX2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSH-SY5Y cells
Positive IP detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25414-1-AP targets AFF2 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag22225

Product name: Recombinant human AFF2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 80-263 aa of BC132683

Sequence: LLTNHSNQNHLVGIPKNSVPQNPNNKNEPSFFPEQKNRIIPPHQDNTHPSAPMPPPSVVILNSTLIHSNRKSKPEWSRDSHNPSTVLASQASGQPNKMQTLTQDQSQAKLEDFFVYPAEQPQIGEVEESNPSAKEDSNPNSSGEDAFKEIFQSNSPEESEFAVQAPGSPLVASSLLAPSSGLSV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 AF4/FMR2 family, member 2
别名 AF4/FMR2 family member 2, FMR2, FMR2P, FRAXE, MRX2
计算分子量 1311 aa, 145 kDa
观测分子量145 kDa
GenBank蛋白编号BC132683
基因名称 AFF2
Gene ID (NCBI) 2334
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP51816
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AFF2 (OMIM* 300806) (also known as FMR2 gene), which encodes AF4/FMR2 family member 2, is a transcriptional factor and RNA-binding protein that plays an important role in transcriptional regulation, RNA splicing, mRNA processing, and nuclear speckle organization . AFF2 is highly conserved and abundantly expressed in human brain, being essential for brain development. Homozygous AFF2 knock-out mice showed abnormal central nervous system synaptic transmission, abnormal excitatory postsynaptic potential, and premature death (PMID: 35431806).

实验方案

Product Specific Protocols
IP protocol for AFF2 antibody 25414-1-APDownload protocol
WB protocol for AFF2 antibody 25414-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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