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验证数据展示

  • WB analysis using 25414-1-AP

    WB analysis using 25414-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25414-1-AP (AFF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of SH-SY5Y using 25414-1-AP

    IP experiment of SH-SY5Y using 25414-1-AP

    IP result of anti-AFF2 (IP:25414-1-AP, 4ug; Detection:25414-1-AP 1:600) with SH-SY5Y cells lysate 1720 ug.

AFF2 Polyclonal antibody

AFF2 Polyclonal Antibody for WB, IP, ELISA
Cat No. 25414-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

AF4/FMR2 family member 2, FMR2, FMR2P, FRAXE, MRX2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSH-SY5Y cells
Positive IP detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25414-1-AP targets AFF2 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag22225

Product name: Recombinant human AFF2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 80-263 aa of BC132683

Sequence: LLTNHSNQNHLVGIPKNSVPQNPNNKNEPSFFPEQKNRIIPPHQDNTHPSAPMPPPSVVILNSTLIHSNRKSKPEWSRDSHNPSTVLASQASGQPNKMQTLTQDQSQAKLEDFFVYPAEQPQIGEVEESNPSAKEDSNPNSSGEDAFKEIFQSNSPEESEFAVQAPGSPLVASSLLAPSSGLSV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 AF4/FMR2 family, member 2
别名 AF4/FMR2 family member 2, FMR2, FMR2P, FRAXE, MRX2
计算分子量 1311 aa, 145 kDa
观测分子量145 kDa
GenBank蛋白编号BC132683
基因名称 AFF2
Gene ID (NCBI) 2334
RRIDAB_3742147
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP51816
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AFF2 (OMIM* 300806) (also known as FMR2 gene), which encodes AF4/FMR2 family member 2, is a transcriptional factor and RNA-binding protein that plays an important role in transcriptional regulation, RNA splicing, mRNA processing, and nuclear speckle organization . AFF2 is highly conserved and abundantly expressed in human brain, being essential for brain development. Homozygous AFF2 knock-out mice showed abnormal central nervous system synaptic transmission, abnormal excitatory postsynaptic potential, and premature death (PMID: 35431806).

实验方案

Product Specific Protocols
IP protocol for AFF2 antibody 25414-1-APDownload protocol
WB protocol for AFF2 antibody 25414-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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