验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, Ramos cells, NIH/3T3 cells, C6 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86131-1-RR targets ADSL in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | ADSL fusion protein Ag7332 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | adenylosuccinate lyase |
| 别名 | AMPS, ASASE, ASL, EC:4.3.2.2 |
| 计算分子量 | 55 kDa |
| 观测分子量 | 55 kDa |
| GenBank蛋白编号 | BC000253 |
| 基因名称 | ADSL |
| Gene ID (NCBI) | 158 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P30566 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Adenylosuccinate lyase (ADSL) is an essential enzyme for de novo purine biosynthesis and the purine nucleotide cycle, where it plays a key role in the regulation of adenosine monophosphate levels in the cells. Given its crucial role in both cellular replication and metabolism, it is not surprising that ADSL has been found dysregulated in several malignancies, including breast and endometrial cancers as well as glioma. (PMID: 33754045)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ADSL antibody 86131-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |