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验证数据展示

  • WB analysis of K-562 using 13727-1-AP

    WB analysis of K-562 using 13727-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13727-1-AP (GRK3 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 13727-1-AP

    IHC staining of mouse brain using 13727-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13727-1-AP (GRK3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 13727-1-AP

    IHC staining of mouse brain using 13727-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13727-1-AP (GRK3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 13727-1-AP

    IHC staining of human colon using 13727-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13727-1-AP (GRK3 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GRK3 Polyclonal antibody

GRK3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13727-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

G protein-coupled receptor kinase 3, EC:2.7.11.15, Beta-ARK-2, Beta-adrenergic receptor kinase 2, ADRBK2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, HL-60 cells
Positive IHC detected inmouse brain tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13727-1-AP targets GRK3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4685

Product name: Recombinant human ADRBK2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-387 aa of BC036797

Sequence: MADLEAVLADVSYLMAMEKSKATPAARASKRIVLPEPSIRSVMQKYLAERNEITFDKIFNQKIGFLLFKDFCLNEINEAVPQVKFYEEIKEYEKLDNEEDRLCRSRQIYDAYIMKELLSCSHPFSKQAVEHVQSHLSKKQVTSTLFQPYIEEICESLRGDIFQKFMESDKFTRFCQWKNVELNIHLTMNEFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMTYAFHTPDKLCFILDLMNGGDLHYHLSQHGVFSEKEMRFYATEIILGLEHMHNRFVVYRDLKPANILLDEHGHARISDLGLACDFSKKKPHASVGTHGYMAPEVLQKGTAYDSSADWFSLGCMLFKLLRG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adrenergic, beta, receptor kinase 2
别名 G protein-coupled receptor kinase 3, EC:2.7.11.15, Beta-ARK-2, Beta-adrenergic receptor kinase 2, ADRBK2
计算分子量 688 aa, 80 kDa
观测分子量 70 kDa, 90 kDa
GenBank蛋白编号BC036797
基因名称 GRK3
Gene ID (NCBI) 157
RRIDAB_2225851
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP35626
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GRK3 (G protein coupled receptor kinase 3), also known as ADRBK2 or BARK2, belongs to the G protein coupled receptor kinases (GRKs) family. GRKs are serine/threonine kinases that regulate a large and diverse class of G-protein coupled receptors (GPCRs). GRKs have been shown to play a critical role in the outcome of a variety of physiological and pathophysiological processes including chemotaxis, signaling, migration, inflammatory gene expression and so on (PMID: 28950947, 29483837). GRK3 was identified as a negative regulator of CXCL12/CXCR4 signaling, and has also been shown to be overexpressed in human prostate cancer and found to promote tumor growth and metastasis by enhancing angiogenesis (PMID: 23935208, 24434559). GRK3 has a molecular mass of 75-80 kDa, and also can be ubiquitinated (PMID: 20596523).

实验方案

Product Specific Protocols
WB protocol for GRK3 antibody 13727-1-APDownload protocol
IHC protocol for GRK3 antibody 13727-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols