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验证数据展示

  • WB analysis using 68034-1-Ig

    WB analysis using 68034-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of LNCaP using 68034-1-Ig

    WB analysis of LNCaP using 68034-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 68034-1-Ig

    WB analysis of A549 using 68034-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 68034-1-Ig

    WB analysis of HeLa using 68034-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of THP-1 using 68034-1-Ig

    WB analysis of THP-1 using 68034-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of human placenta using 68034-1-Ig

    WB analysis of human placenta using 68034-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

ADPGK Monoclonal antibody

ADPGK Monoclonal Antibody for WB, ELISA
Cat No. 68034-1-Ig

产品说明书

CloneNo. 2B4B8

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, ELISA

别名

2610017G09Rik, ADP dependent glucokinase, ADP GK, ADPGK, DKFZp434B195, RbBP 35

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, human placenta tissue, LNCaP cells, THP-1 cells, Jurkat cells, K-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68034-1-Ig targets ADPGK in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag31242

Product name: Recombinant human ADPGK protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 362-497 aa of BC006112

Sequence: ILKEHGRSKSRASDLTRIHFHTLVYHILATVDGHWANQLAAVAAGARVAGTQACATETIDTSRVSLRAPQEFMTSHSEAGSRIVLNPNKPVVEWHREGISFHFTPVLVCKDPIRTVGLGDAISAEGLFYSEVHPHY

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ADP-dependent glucokinase
别名 2610017G09Rik, ADP dependent glucokinase, ADP GK, ADPGK, DKFZp434B195, RbBP 35
计算分子量 497 aa, 54 kDa
观测分子量51 kDa
GenBank蛋白编号BC006112
基因名称 ADPGK
Gene ID (NCBI) 83440
RRIDAB_2918776
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9BRR6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADP-dependent glucokinase (ADPGK) has frst been described 1994 in hyperthermophilic archaea as a novel glucose-phosphorylating enzyme dependent on ADP (adenosine diphosphate) instead of ATP (adenosine triphosphate). Highest ADPGK expression is found in immune cells of both myeloid and lymphoid lineages. Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency (By similarity).

实验方案

Product Specific Protocols
WB protocol for ADPGK antibody 68034-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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