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验证数据展示

  • WB analysis of mouse brain using 51092-1-AP (same clone as 51092-1-PBS)

    WB analysis of mouse brain using 51092-1-AP (same clone as 51092-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 51092-1-AP (ADORA2A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51092-1-PBS in a different storage buffer formulation.

  • WB analysis using 51092-1-AP (same clone as 51092-1-PBS)

    WB analysis using 51092-1-AP (same clone as 51092-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 51092-1-AP (ADORA2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51092-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 51092-1-AP (same clone as 51092-1-PBS)

    IHC staining of mouse brain using 51092-1-AP (same clone as 51092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 51092-1-AP (ADORA2A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51092-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 51092-1-AP (same clone as 51092-1-PBS)

    IHC staining of mouse brain using 51092-1-AP (same clone as 51092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 51092-1-AP (ADORA2A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51092-1-PBS in a different storage buffer formulation.

ADORA2A Polyclonal antibody, PBS Only

ADORA2A Polyclonal Antibody for IHC, Indirect ELISA
Cat No. 51092-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, Indirect ELISA

别名

adenosine A2a receptor, Adenosine receptor A2a, ADORA2, RDC8

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

51092-1-PBS targets ADORA2A in IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0470

Product name: Recombinant human ADORA2A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 300-412 aa of BC013780

Sequence: RKIIRSHVLRQQEPFKAAGTSARVLAAHGSDGEQVSLRLNGHPPGVWANGSAPHPERRPNGYALGLVSGGSAQESQGNTGLPDVELLSHELKGVCPEPPGLDDPLAQDGAGVS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adenosine A2a receptor
别名 adenosine A2a receptor, Adenosine receptor A2a, ADORA2, RDC8
计算分子量 412 aa, 45 kDa
观测分子量 38 kDa, 40-45 kDa
GenBank蛋白编号BC013780
基因名称 ADORA2A
Gene ID (NCBI) 135
RRIDAB_2881245
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP29274
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Adenosine (ADO) is a retaliatory metabolite that is expressed in conditions of injury or stress. Its physiological functions are mediated through interaction with four specific transmembrane receptors called ADORA1, ADORA2A, ADORA2B, and ADORA3 (PMID:23994158). ADORA2A (adenosine A2A receptor) has been shown to dampen inflammatory responses. ADORA2A signaling has been discovered on polymorphonuclear neutrophils (PMNs) (PMID:36009485).

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