验证数据展示
经过测试的应用
| Positive WB detected in | mouse eye tissue |
| Positive IHC detected in | mouse brain tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20332-1-AP targets Adenosine A1 Receptor in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, FC (Intra) |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag14092 Product name: Recombinant human ADORA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 184-243 aa of BC026340 Sequence: NFFVWVLPPLLLMVLIYLEVFYLIRKQLNKKVSASSGDPQKYYGKELKIAKSLALILFLF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | adenosine A1 receptor |
| 别名 | Adenosine receptor A1, ADORA1, RDC7 |
| 计算分子量 | 326 aa, 37 kDa |
| 观测分子量 | 39 kDa |
| GenBank蛋白编号 | BC026340 |
| 基因名称 | Adenosine A1 Receptor |
| Gene ID (NCBI) | 134 |
| RRID | AB_2878673 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P30542 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ADORA1, also named as RDC7, is a receptor for adenosine. It is mediated by G proteins which inhibit adenylyl cyclase. Adenosine is an important mediator of ethanol intoxication and exerts some of its effects via ADORA1 in the central nervous system.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Adenosine A1 Receptor antibody 20332-1-AP | Download protocol |
| IHC protocol for Adenosine A1 Receptor antibody 20332-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Biol Toxicol Adenosine kinase protects against acetaminophen-induced acute liver injury by activating autophagy in hepatocytes | ||
PLoS One Discovery of a potent, selective, and tumor-suppressing antibody antagonist of adenosine A2A receptor | ||
J Orthop Translat CD73 alleviates osteoarthritis by maintaining anabolism and suppressing catabolism of chondrocytes extracellular matrix | ||
Am J Physiol Cell Physiol Abnormal purine metabolism in nasal epithelial cells affects allergic rhinitis by regulating Th17/Treg cells |
