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ADHFE1 Polyclonal antibody

ADHFE1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 19359-1-AP

Print datasheet

Synonyms

ADH8, ADHFE1, HMFT2263, HOT

验证数据展示

  • WB analysis of mouse heart using 19359-1-AP

    WB analysis of mouse heart using 19359-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 19359-1-AP (ADHFE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 19359-1-AP

    IHC staining of human colon using 19359-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19359-1-AP (ADHFE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 19359-1-AP

    IHC staining of human colon using 19359-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19359-1-AP (ADHFE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 19359-1-AP

    IHC staining of human colon cancer using 19359-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19359-1-AP (ADHFE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 19359-1-AP

    IHC staining of human colon cancer using 19359-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19359-1-AP (ADHFE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse heart using 19359-1-AP

    IP experiment of mouse heart using 19359-1-AP

    IP result of anti-ADHFE1 (IP:19359-1-AP, 4ug; Detection:19359-1-AP 1:300) with mouse heart tissue lysate 4000ug.

  • IHC staining of human liver using 19359-1-AP

    IHC staining of human liver using 19359-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19359-1-AP (ADHFE1 Antibody) at dilution of 1:50(under 10x lens).

  • IHC staining of human liver using 19359-1-AP

    IHC staining of human liver using 19359-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19359-1-AP (ADHFE1 Antibody) at dilution of 1:50(under 40x lens).

经过测试的应用

Positive WB detected inmouse heart tissue, mouse kidney tissue, rat kidney tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman colon tissue, human colon cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 19359-1-AP is ADHFE1 Fusion Protein expressed in E. coli.

Tested Applications IHC, IP, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen ADHFE1 fusion protein Ag6803 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name alcohol dehydrogenase, iron containing, 1
Synonyms ADH8, ADHFE1, HMFT2263, HOT
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC064634
Gene Symbol ADHFE1
Gene ID (NCBI) 137872
RRIDAB_10644280
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IWW8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ADHFE1 antibody 19359-1-APDownload protocol
IHC protocol for ADHFE1 antibody 19359-1-APDownload protocol
IP protocol for ADHFE1 antibody 19359-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols