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验证数据展示

  • IP experiment of mouse liver using 18897-1-AP (same clone as 18897-1-PBS)

    IP experiment of mouse liver using 18897-1-AP (same clone as 18897-1-PBS)

    IP result of anti-ADH1C (IP:18897-1-AP, 4ug; Detection:18897-1-AP 1:1000) with mouse liver tissue lysate 4000 ug. This data was developed using the same antibody clone with 18897-1-PBS in a different storage buffer formulation.

  • WB analysis using 18897-1-AP (same clone as 18897-1-PBS)

    WB analysis using 18897-1-AP (same clone as 18897-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18897-1-AP (ADH1C antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18897-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 18897-1-AP (same clone as 18897-1-PBS)

    IHC staining of human lung cancer using 18897-1-AP (same clone as 18897-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18897-1-AP (ADH1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18897-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ADH1C antibody included
  • IHC staining of human colon using 18897-1-AP (same clone as 18897-1-PBS)

    IHC staining of human colon using 18897-1-AP (same clone as 18897-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18897-1-AP (ADH1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18897-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ADH1C antibody included
  • IHC staining of rat liver using 18897-1-AP (same clone as 18897-1-PBS)

    IHC staining of rat liver using 18897-1-AP (same clone as 18897-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 18897-1-AP (ADH1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18897-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ADH1C antibody included
  • IF Staining of HepG2 using 18897-1-AP (same clone as 18897-1-PBS)

    IF Staining of HepG2 using 18897-1-AP (same clone as 18897-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ADH1C antibody (18897-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 18897-1-PBS in a different storage buffer formulation.

ADH1C Polyclonal antibody, PBS Only

ADH1C Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 18897-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

EC:1.1.1.1, ADH3

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ADH1C Ready-To-Use IHC Kit. ADH1C primary antibody included.

产品信息

18897-1-PBS targets ADH1C in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag13528

Product name: Recombinant human ADH1C protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-374 aa of BC062476

Sequence: MSTAGKVIKCKAAVLWELKKPFSIEEVEVAPPKAHEVRIKMVAAGICRSDEHVVSGNLVTPLPVILGHEAAGIVESVGEGVTTVKPGDKVIPLFTPQCGKCRICKNPESNYCLKNDLGNPRGTLQDGTRRFTCSGKPIHHFVGVSTFSQYTVVDENAVAKIDAASPLEKVCLIGCGFSTGYGSAVKVAKVTPGSTCAVFGLGGVGLSVVMGCKAAGAARIIAVDINKDKFAKAKELGATECINPQDYKKPIQEVLKEMTDGGVDFSFEVIGQLDTMMASLLCCHEACGTSVIVGVPPDSQNLSINPMLLLTGRTWKGAIFGGFKSKESVPKLVADFMAKKFSLDALITNVLPFEKINEGFDLLRSGKSIRTVLT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 alcohol dehydrogenase 1C (class I), gamma polypeptide
别名 EC:1.1.1.1, ADH3
计算分子量 375 aa, 40 kDa
观测分子量 37-45 kDa
GenBank蛋白编号BC062476
基因名称 ADH1C
Gene ID (NCBI) 126
RRIDAB_10596950
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP00326
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ADH1C(Alcohol dehydrogenase 1C) is also named as ADH3 and belongs to the zinc-containing alcohol dehydrogenase family.It catalyzes the rate-limiting step for ethanol metabolism: the oxidation of alcohol to acetaldehyde.The ADH1C gene is active in intestine and kidney in fetal and early postnatal life, and persists in the stomach and liver in adult life(PMID:4748759).

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