验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, K-562 cells, mouse kidney tissue |
| Positive IP detected in | NIH/3T3 cells |
Planning an IHC experiment? We recommend our IHCeasy® Gamma Adducin Ready-To-Use IHC Kit. Gamma Adducin primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
产品信息
17585-1-AP targets Gamma Adducin in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag11318 Product name: Recombinant human ADD3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 364-707 aa of BC062559 Sequence: WKVGEIEFEGLMRTLDNLGYRTGYAYRHPLIREKPRHKSDVEIPATVTAFSFEDDTVPLSPLKYMAQRQQREKTRWLNSPNTYMKVNVPEESRNGETSPRTKITWMKAEDSSKVSGGTPIKIEDPNQFVPLNTNPNEVLEKRNKIREQNRYDLKTAGPQSQLLAGIVVDKPPSTMQFEDDDHGPPAPPNPFSHLTEGELEEYKRTIERKQQGLEDAEQELLSDDASSVSQIQSQTQSPQNVPEKLEENHELFSKSFISMEVPVMVVNGKDDMHDVEDELAKRVSRLSTSTTIENIEITIKSPEKIEEVLSPEGSPSKSPSKKKKKFRTPSFLKKNKKKEKVEA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | adducin 3 (gamma) |
| 别名 | ADD3, ADDL, adducin 3 (gamma), Adducin like protein 70, Gamma adducin |
| 计算分子量 | 76 kDa, 79 kDa |
| 观测分子量 | 80 kDa, 90 kDa |
| GenBank蛋白编号 | BC062559 |
| 基因名称 | Gamma Adducin |
| Gene ID (NCBI) | 120 |
| RRID | AB_2223579 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UEY8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Gamma Adducin antibody 17585-1-AP | Download protocol |
| IP protocol for Gamma Adducin antibody 17585-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Mol Cell Biol QKI-5 regulates the alternative splicing of cytoskeletal gene ADD3 in lung cancer. | ||
Stem Cell Res Ther Knockdown of long noncoding RNA SAN rejuvenates aged adipose-derived stem cells via miR-143-3p/ADD3 axis
| ||
PLoS One Dysferlin and other non-red cell proteins accumulate in the red cell membrane of diamond-blackfan anemia patients. | ||
Cell Genom Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders |
