验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, mouse testis tissue |
| Positive IHC detected in | human ovary cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
10791-1-AP targets Alpha Adducin in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | rat, bovine |
| 免疫原 |
CatNo: Ag1245 Product name: Recombinant human ADD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-400 aa of BC013393 Sequence: MNGDSRAAVVTSPPPTTAPHKERYFDRVDENNPEYLRERNMAPDLRQDFNMMEQKKRVSMILQSPAFCEELESMIQEQFKKGKNPTGLLALQQIADFMTTNVPNVYPAAPQGGMAALNMSLGMVTPVNDLRGSDSIAYDKGEKLLRCKLAAFYRLADLFGWSQLIYNHITTRVNSEQEHFLIVPFGLLYSEVTASSLVKINLQGDIVDRGSTNLGVNQAGFTLHSAIYAARPDVKCVVHIHTPAGAAVSAMKCGLLPISPEALSLGEVAYHDYHGILVDEEEKVLIQKNLGPKSKVLILRNHGLVSVGESVEEAFYYIHNLVVACEIQVRTLASAGGPDNLVLLNPEKYKAKSRSPGSPVGEGTGSPPKWQIGEQEFEALMRMLDNLGYRTSSSGRRQDG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | adducin 1 (alpha) |
| 别名 | ADD1, Alpha-adducin, adducin 1 (alpha), ADDA, ADD 1 |
| 计算分子量 | 81 kDa, 84 kDa, 70 kDa, 73 kDa, 56 kDa |
| 观测分子量 | 115-120 kDa |
| GenBank蛋白编号 | BC013393 |
| 基因名称 | Alpha Adducin |
| Gene ID (NCBI) | 118 |
| RRID | AB_2257952 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P35611 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Alpha Adducin antibody 10791-1-AP | Download protocol |
| IHC protocol for Alpha Adducin antibody 10791-1-AP | Download protocol |
| WB protocol for Alpha Adducin antibody 10791-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Mol Sci Decoding the Synaptic Proteome with Long-Term Exposure to Midazolam during Early Development. | ||
Mol Biol Rep Depletion of serum-derived exosomes aggravates heat stress-induced damage of bovine mammary epithelial cells |
