验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, mouse brain tissue, SKOV-3 cells |
| Positive IP detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| CoIP | See 1 publications below |
产品信息
14616-1-AP targets ADCY6 in WB, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag6218 Product name: Recombinant human ADCY6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 310-655 aa of BC064923 Sequence: AEVSQRQAFQETRGYIQARLHLQHENRQQERLLLSVLPQHVAMEMKEDINTKKEDMMFHKIYIQKHDNVSILFADIEGFTSLASQCTAQELVMTLNELFARFDKLAAENHCLRIKILGDCYYCVSGLPEARADHAHCCVEMGVDMIEAISLVREVTGVNVNMRVGIHSGRVHCGVLGLRKWQFDVWSNDVTLANHMEAGGRAGRIHITRATLQYLNGDYEVEPGRGGERNAYLKEQHIETFLILGASQKRKEEKAMLAKLQRTRANSMEGLMPRWVPDRAFSRTKDSKAFRQMGIDDSSKDNRGTQDALNPEDEVDEFLSRAIDARSIDQLRKDHVRRFLLTFQRE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | adenylate cyclase 6 |
| 别名 | AC6, ATP pyrophosphate-lyase 6, Ca(2+)-inhibitable adenylyl cyclase, EC:4.6.1.1 |
| 计算分子量 | 131 kDa |
| 观测分子量 | 130-150 kDa |
| GenBank蛋白编号 | BC064923 |
| 基因名称 | ADCY6 |
| Gene ID (NCBI) | 112 |
| RRID | AB_2242343 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43306 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Adenylate cyclase 6 (ADCY6) belongs to the membrane-bound adenylate cyclase family. Increasing evidence has proven that ADCY6 is involved in pathophysiological processes of the body, such as the stability of cardiovascular system function, glucose and lipid metabolism, and even carcinogenesis.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for ADCY6 antibody 14616-1-AP | Download protocol |
| WB protocol for ADCY6 antibody 14616-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Multiple cAMP/PKA complexes at the STIM1 ER/PM junction specified by E-Syt1 and E-Syt2 reciprocally gates ANO1 (TMEM16A) via Ca2 | ||
Nat Commun Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH) | ||
J Cell Biol Sonic Hedgehog activates prostaglandin signaling to stabilize primary cilium length | ||
Mol Pharmacol Activity of adenylyl cyclase type 6 is suppressed by direct binding of the cytoskeletal protein 4.1G. | ||
Pathol Oncol Res Knockdown of GPSM1 Inhibits the Proliferation and Promotes the Apoptosis of B-Cell Acute Lymphoblastic Leukemia Cells by Suppressing the ADCY6-RAPGEF3-JNK Signaling Pathway. |
