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ADCY1 Polyclonal antibody

ADCY1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IHC, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 55067-1-AP

Print datasheet

Synonyms

AC1, ADCY1, adenylate cyclase 1 (brain), Adenylate cyclase type 1, Adenylate cyclase type I, Adenylyl cyclase 1, ATP pyrophosphate lyase 1

验证数据展示

  • WB analysis of SH-SY5Y using 55067-1-AP

    WB analysis of SH-SY5Y using 55067-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55067-1-AP (ADCY1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 55067-1-AP

    IHC staining of mouse brain using 55067-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55067-1-AP (ADCY1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 55067-1-AP

    IHC staining of mouse brain using 55067-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55067-1-AP (ADCY1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inSH-SY5Y cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

55067-1-AP targets ADCY1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB,ELISA
Cited ApplicationsIF
Tested Reactivity human, mouse
Cited Reactivitypig
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name adenylate cyclase 1 (brain)
Synonyms AC1, ADCY1, adenylate cyclase 1 (brain), Adenylate cyclase type 1, Adenylate cyclase type I, Adenylyl cyclase 1, ATP pyrophosphate lyase 1
Calculated Molecular Weight 123 kDa
Observed Molecular Weight 123 kDa
GenBank Accession NumberNM_021116
Gene Symbol ADCY1
Gene ID (NCBI) 107
RRIDAB_10858391
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ08828
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADCY1 belongs to the adenylyl cyclase class-4/guanylyl cyclase family. It is a calmodulin-sensitive adenylyl cyclase. ADCY1 may be involved in regulatory processes in the central nervous system. It may play a role in memory acquisition and learning. The antibody is specific to ADCY1.

实验方案

Product Specific Protocols
WB protocol for ADCY1 antibody 55067-1-APDownload protocol
IHC protocol for ADCY1 antibody 55067-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigIF

Front Pharmacol

Inhibition of adenylyl cyclase 1 by ST034307 inhibits IP3-evoked changes in sino-atrial node beat rate

Authors - Samuel J. Bose
View Article