验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 1 publications below |
产品信息
55067-1-AP targets ADCY1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, pig |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | adenylate cyclase 1 (brain) |
| 别名 | Adenylyl cyclase 1, AC1, ADCY 1, Adenylate Cyclase 1, Adenylyl Cyclase Subtype 1 |
| 计算分子量 | 123 kDa |
| 观测分子量 | 123 kDa |
| GenBank蛋白编号 | NM_021116 |
| 基因名称 | ADCY1 |
| Gene ID (NCBI) | 107 |
| RRID | AB_10858391 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q08828 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ADCY1 belongs to the adenylyl cyclase class-4/guanylyl cyclase family. It is a calmodulin-sensitive adenylyl cyclase. ADCY1 may be involved in regulatory processes in the central nervous system. It may play a role in memory acquisition and learning. The antibody is specific to ADCY1.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ADCY1 antibody 55067-1-AP | Download protocol |
| WB protocol for ADCY1 antibody 55067-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Pharmacol Inhibition of adenylyl cyclase 1 by ST034307 inhibits IP3-evoked changes in sino-atrial node beat rate | ||
Diabetes Visceral adipocyte-derived extracellular vesicle miR-27a-5p elicits glucose intolerance by inhibiting pancreatic β-cell insulin secretion | ||
Proc Natl Acad Sci U S A KCTD1 regulation of Adenylyl cyclase type 5 adjusts striatal cAMP signaling |
