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验证数据展示

  • WB analysis of mouse brain using 17579-1-AP

    WB analysis of mouse brain using 17579-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17579-1-AP (ADCK5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 17579-1-AP

    IHC staining of human ovary cancer using 17579-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17579-1-AP (ADCK5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ADCK5 Polyclonal antibody

ADCK5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17579-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

EC:2.7.11.-, Uncharacterized aarF domain-containing protein kinase 5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17579-1-AP targets ADCK5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag11094

Product name: Recombinant human ADCK5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-169 aa of BC031570

Sequence: MRAHAAALGVQDYLLFAEMLMQRPVRLGQLWGSHLLSREEAAYMVDMARERFEAVMAVLRELPRPMLLVLRNINTVRAINVALGAPVDRYFLMAKRAVRGWSRLAGATYRGVYGTSLLRHAKVVWEMLKFEVALRLETLAMRLTALLARALVHLSLVPPAEELYQYLET

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 aarF domain containing kinase 5
别名 EC:2.7.11.-, Uncharacterized aarF domain-containing protein kinase 5
计算分子量 66 kDa
观测分子量 66 kDa
GenBank蛋白编号BC031570
基因名称 ADCK5
Gene ID (NCBI) 203054
RRIDAB_2242313
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ3MIX3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADCK5 (AarF Domain Containing Kinase 5) is a protein kinase belonging to the ADCK protein family. Named for its AarF domain, this family plays important roles in cellular energy metabolism and mitochondrial function regulation. ADCK5 is primarily expressed in human tissues and participates in the biosynthesis of Coenzyme Q (CoQ). CoQ is a critical component of the mitochondrial electron transport chain, essential for cellular energy production. ADCK5 contains ubiquitination modification sites, and the experimentally detected molecular weight may range between 65-75 kDa.

实验方案

Product Specific Protocols
IHC protocol for ADCK5 antibody 17579-1-APDownload protocol
WB protocol for ADCK5 antibody 17579-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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