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验证数据展示

  • IP experiment of mouse brain using 13621-1-AP

    IP experiment of mouse brain using 13621-1-AP

    IP result of anti-ADAT2 (IP:13621-1-AP, 4ug; Detection:13621-1-AP 1:300) with mouse brain tissue lysate 3000 ug.

  • WB analysis of A431 using 13621-1-AP

    WB analysis of A431 using 13621-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 13621-1-AP (ADAT2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human hepatocirrhosis using 13621-1-AP

    IHC staining of human hepatocirrhosis using 13621-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 13621-1-AP (ADAT2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human hepatocirrhosis using 13621-1-AP

    IHC staining of human hepatocirrhosis using 13621-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 13621-1-AP (ADAT2 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of A431 using 13621-1-AP

    IF Staining of A431 using 13621-1-AP

    Immunofluorescent analysis of A431 cells using 13621-1-AP (ADAT2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human colon cancer using 13621-1-AP

    IHC staining of human colon cancer using 13621-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13621-1-AP (ADAT2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 13621-1-AP

    IHC staining of human colon cancer using 13621-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13621-1-AP (ADAT2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ADAT2 Polyclonal antibody

ADAT2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 13621-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

tRNA-specific adenosine deaminase 2, TAD2, EC:3.5.4.33, Deaminase domain-containing protein 1, DEADC1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, mouse brain tissue, mouse pancreas tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman hepatocirrhosis tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13621-1-AP targets ADAT2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原

CatNo: Ag4529

Product name: Recombinant human ADAT2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 33-193 aa of BC037955

Sequence: HMAKEALENTEVPVGCLMVYNNEVVGKGRNEVNQTKNATRHAEMVAIDQVLDWCRQSGKSPSEVFEHTVLYVTVEPCIMCAAALRLMKIPLVVYGCQNERFGGCGSVLNIASADLPNTGRPFQCIPGYRAEEAVEMLKTFYKQENPNAPKSKVRKKECQKS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adenosine deaminase, tRNA-specific 2, TAD2 homolog (S. cerevisiae)
别名 tRNA-specific adenosine deaminase 2, TAD2, EC:3.5.4.33, Deaminase domain-containing protein 1, DEADC1
计算分子量 191 aa, 21 kDa
观测分子量 25 kDa
GenBank蛋白编号BC037955
基因名称 ADAT2
Gene ID (NCBI) 134637
RRIDAB_10642142
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z6V5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADAT2(tRNA-specific adenosine deaminase 2) is also named as DEADC1 and Belongs to the cytidine and deoxycytidylate deaminase family. It is a 24-kDa molecular mass protein that harbors all the conserved motifs required for deamination. ADAT2 and ADAT3 can function as a heterodimer which catalyses inosine formation at the wobble position (position 34) in eukaryotic tRNAs(PMID:12457566). It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for ADAT2 antibody 13621-1-APDownload protocol
IHC protocol for ADAT2 antibody 13621-1-APDownload protocol
IP protocol for ADAT2 antibody 13621-1-APDownload protocol
WB protocol for ADAT2 antibody 13621-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nucleic Acids Res

The structure of the mouse ADAT2/ADAT3 complex reveals the molecular basis for mammalian tRNA wobble adenosine-to-inosine deamination.

Authors - Elizabeth Ramos-Morales
View Article
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