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ADAT2 Polyclonal antibody

ADAT2 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 13621-1-AP

Print datasheet

Synonyms

ADAT2, DEADC1, dJ20N2, TAD2

验证数据展示

  • IP experiment of mouse brain using 13621-1-AP

    IP experiment of mouse brain using 13621-1-AP

    IP result of anti-ADAT2 (IP:13621-1-AP, 4ug; Detection:13621-1-AP 1:300) with mouse brain tissue lysate 3000 ug.

  • WB analysis of A431 using 13621-1-AP

    WB analysis of A431 using 13621-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 13621-1-AP (ADAT2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human hepatocirrhosis using 13621-1-AP

    IHC staining of human hepatocirrhosis using 13621-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 13621-1-AP (ADAT2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human hepatocirrhosis using 13621-1-AP

    IHC staining of human hepatocirrhosis using 13621-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 13621-1-AP (ADAT2 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of A431 using 13621-1-AP

    IF Staining of A431 using 13621-1-AP

    Immunofluorescent analysis of A431 cells using 13621-1-AP (ADAT2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human colon cancer using 13621-1-AP

    IHC staining of human colon cancer using 13621-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13621-1-AP (ADAT2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 13621-1-AP

    IHC staining of human colon cancer using 13621-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13621-1-AP (ADAT2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA431 cells, mouse brain tissue, mouse pancreas tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman hepatocirrhosis tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13621-1-AP targets ADAT2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen ADAT2 fusion protein Ag4529 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name adenosine deaminase, tRNA-specific 2, TAD2 homolog (S. cerevisiae)
Synonyms ADAT2, DEADC1, dJ20N2, TAD2
Calculated Molecular Weight 191 aa, 21 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC037955
Gene Symbol ADAT2
Gene ID (NCBI) 134637
RRIDAB_10642142
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z6V5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADAT2(tRNA-specific adenosine deaminase 2) is also named as DEADC1 and Belongs to the cytidine and deoxycytidylate deaminase family. It is a 24-kDa molecular mass protein that harbors all the conserved motifs required for deamination. ADAT2 and ADAT3 can function as a heterodimer which catalyses inosine formation at the wobble position (position 34) in eukaryotic tRNAs(PMID:12457566). It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for ADAT2 antibody 13621-1-APDownload protocol
IHC protocol for ADAT2 antibody 13621-1-APDownload protocol
IF protocol for ADAT2 antibody 13621-1-APDownload protocol
IP protocol for ADAT2 antibody 13621-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nucleic Acids Res

The structure of the mouse ADAT2/ADAT3 complex reveals the molecular basis for mammalian tRNA wobble adenosine-to-inosine deamination.

Authors - Elizabeth Ramos-Morales
View Article