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验证数据展示

  • WB analysis using 26836-1-AP

    WB analysis using 26836-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 26836-1-AP (ADAMTS7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 26836-1-AP

    IHC staining of mouse brain using 26836-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26836-1-AP (ADAMTS7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ADAMTS7 Polyclonal antibody

ADAMTS7 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26836-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA and More (1)

别名

ADAM TS 7, ADAM TS7, ADAMTS 7, ADAMTS7, COMPase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26836-1-AP targets ADAMTS7 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag25218

Product name: Recombinant human ADAMTS7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1507-1686 aa of BC061631

Sequence: PAKPPAHRPCGAQPCLSWYTSSWRECSEACGGGEQQRLVTCPEPGLCEEALRPNTTRPCNTHPCTQWVVGPWGQCSGPCGGGVQRRLVKCVNTQTGLPEEDSDQCGHEAWPESSRPCGTEDCEPVEPPRCERDRLSFGFCETLRLLGRCQLPTIRTQCCRSCSPPSHGAPSRGHQRVARR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ADAM metallopeptidase with thrombospondin type 1 motif, 7
别名 ADAM TS 7, ADAM TS7, ADAMTS 7, ADAMTS7, COMPase
计算分子量 1686 aa, 184 kDa
观测分子量 184 kDa
GenBank蛋白编号BC061631
基因名称 ADAMTS7
Gene ID (NCBI) 11173
RRIDAB_2880653
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UKP4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ADAMTS7 antibody 26836-1-APDownload protocol
IHC protocol for ADAMTS7 antibody 26836-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,IF

Eur J Med Res

Diagnostic value of serum COMP and ADAMTS7 for intervertebral disc degeneration

Authors - Jing-Yu Ding
View Article
humanWB

J Cancer

ADAMTS7 Enhances Gastric Cancer Growth and Metastasis by Triggering the NF-κB Signaling Pathway

Authors - Shun Chen
  • KD Validated
View Article