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验证数据展示

  • IF Staining of NIH/3T3 using 24934-1-AP (same clone as 24934-1-PBS)

    IF Staining of NIH/3T3 using 24934-1-AP (same clone as 24934-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using ADAMTS12 antibody (24934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 24934-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse placenta using 24934-1-AP (same clone as 24934-1-PBS)

    WB analysis of mouse placenta using 24934-1-AP (same clone as 24934-1-PBS)

    mouse placenta tissue were subjected to SDS PAGE followed by western blot with 24934-1-AP (ADAMTS12 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24934-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ADAMTS12 Polyclonal antibody, PBS Only

ADAMTS12 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 24934-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

24934-1-PBS targets ADAMTS12 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag20712

Product name: Recombinant human ADAMTS12 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-229 aa of BC058841

Sequence: MPCAQRSWLANLSVVAQLLNFGALCYGRQPQPGPVRFPDRRQEHFIKGLPEYHVVGPVRVDASGHFLSYGLHYPITSSRRKRDLDGSEDWVYYRISHEEKDLFFNLTVNQGFLSNSYIMEKRYGNLSHVKMMASSAPLCHLSGTVLQQGTRVGTAALSACHGLTGFFQLPHGDFFIEPVKKHPLVEGGYHPHIVYRRQKVPETKEPTCGLKGIVTHMSSWVEESVLFFW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ADAM metallopeptidase with thrombospondin type 1 motif, 12
别名
计算分子量 178 kDa
观测分子量 155 kDa
GenBank蛋白编号BC058841
基因名称 ADAMTS12
Gene ID (NCBI) 81792
RRIDAB_2879806
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP58397
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ADAMTS12(A disintegrin and metalloproteinase with thrombospondin motifs 12) is also named as AT12, ADAM-TS12, PRO4389 and belongs to the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. It may play roles in pulmonary cells during fetal development or in tumor processes through its proteolytic activity or as a molecule potentially involved in regulation of cell adhesion. ADAMTS7 and ADAMTS12 appear to be potent negative regulators of endplate cartilage development(PMID:22247065). This protein has 3 isoforms produced by alternative splicing. The full length protein has 14 glycosylation sites.

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