验证数据展示
经过测试的应用
| Positive WB detected in | BxPC-3 cells, mouse pancreas tissue |
| Positive IHC detected in | human tonsillitis tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23778-1-AP targets ADAM8 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | ADAM8 fusion protein Ag17262 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ADAM metallopeptidase domain 8 |
| 别名 | ADAM metallopeptidase domain 8, ADAM8, ADAM8 protein, CD156, MS2 |
| 计算分子量 | 824 aa, 89 kDa |
| 观测分子量 | 65 kDa |
| GenBank蛋白编号 | BC115404 |
| 基因名称 | ADAM8 |
| Gene ID (NCBI) | 101 |
| RRID | AB_2879321 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P78325 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ADAMs are a large family of transmembrane proteins that are involved in proteolysis, making them candidates for mediating the remodeling of the extracellular matrix(ECM). ADAM8, one of the members of the ADAM family, is overexpressed in various human tumors. It has been shown previously that hypoxia influences the expression of some ADAM proteins. The protein found to be expressed in two active forms(90 kDa and 60 kDa): potential shedding protein and remnant protein in the pancreatic tissues under chronic pancreatitis and pancreatic cancer(PMID:18566576). The full length protein has a signal peptide and four glycosylation sites. This antibody is specific to ADAM8.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ADAM8 antibody 23778-1-AP | Download protocol |
| IHC protocol for ADAM8 antibody 23778-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Neurosci Matrix metalloprotease-mediated cleavage of neural glial-related cell adhesion molecules activates quiescent olfactory stem cells via EGFR. | ||
Exp Anim Suppression of ADAM8 attenuates angiotensin II-induced cardiac fibrosis and endothelial-mesenchymal transition via inhibiting TGF-β1/Smad2/Smad3 pathways.
| ||
J Healthc Eng ADAM8 Activates NLRP3 Inflammasome to Promote Cerebral Ischemia-Reperfusion Injury. | ||
Pathol Oncol Res High ADAM8 expression is associated with poor prognosis in patients with hepatocellular carcinoma. | ||
Int J Mol Med ADAM17 promotes lymph node metastasis in gastric cancer via activation of the Notch and Wnt signaling pathways. | ||
Nat Med Translation control of the immune checkpoint in cancer and its therapeutic targeting. |
