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验证数据展示

  • IHC staining of human lung cancer using 21434-1-AP

    IHC staining of human lung cancer using 21434-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21434-1-AP (ADAM21 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 21434-1-AP

    IHC staining of human lung cancer using 21434-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21434-1-AP (ADAM21 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ADAM21 Polyclonal antibody

ADAM21 Polyclonal Antibody for IHC, ELISA
Cat No. 21434-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, ELISA

别名

ADAM 21, ADAM21, ADAM31

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21434-1-AP targets ADAM21 in IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human
免疫原 ADAM21 fusion protein Ag16042 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ADAM metallopeptidase domain 21
别名 ADAM 21, ADAM21, ADAM31
计算分子量 722 aa, 81 kDa
GenBank蛋白编号BC109024
基因名称 ADAM21
Gene ID (NCBI) 8747
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UKJ8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADAM21 is also present in growing axonal tracts during postnatal development and in growing primary olfactory axons in adults. In the olfactory nerve layer, ADAM21 often, but not always, colocalizes with OMP, a marker of mature olfactory neurons, but is not colocalized with the immature marker βIII-tubulin. The exclusive expression of ADAM21 in human testis and its sequence similarity with the fertilins suggest that it is also expressed on sperm cells and involved in sperm maturation and/or fertilization(PMID:9469942).