验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, PC-3 cells, mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20755-1-AP targets ADAM20 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | ADAM20 fusion protein Ag14493 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ADAM metallopeptidase domain 20 |
| 别名 | ADAM 20, ADAM20 |
| 计算分子量 | 776 aa, 87 kDa |
| 观测分子量 | 70-80 kDa |
| GenBank蛋白编号 | BC025378 |
| 基因名称 | ADAM20 |
| Gene ID (NCBI) | 8748 |
| RRID | AB_3669344 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43506 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ADAM20 is the functional equivalent of sperm fertilin-alpha (ADAM1), since that gene is nonfunctional in human. And northern blot analysis detected the 2.8-kb ADAM20 mRNA only in testis. The exclusive expression of ADAM20 in human testis and their sequence similarity with the fertilins suggest that it is expressed on sperm cells and involved in sperm maturation and/or fertilization(PMID:9469942). The full length protein has a singnal peptide, propeptide and 6 glycosylation sites.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ADAM20 antibody 20755-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |