验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue, HepG2 cells, human skeletal muscle tissue |
| Positive IHC detected in | human medulloblastoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12302-1-AP targets ACYP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag2948 Product name: Recombinant human ACYP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-99 aa of BC012290 Sequence: MSTAQSLKSVDYEVFGRVQGVCFRMYTEDEARKIGVVGWVKNTSKGTVTGQVQGPEDKVNSMKSWLSKVGSPSSRIDRTNFSNEKTISKLEYSNFSIRY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | acylphosphatase 2, muscle type |
| 别名 | Acylphosphatase-2, ACYM, ACYP, EC:3.6.1.7 |
| 计算分子量 | 99 aa, 11 kDa |
| 观测分子量 | 11 kDa |
| GenBank蛋白编号 | BC012290 |
| 基因名称 | ACYP2 |
| Gene ID (NCBI) | 98 |
| RRID | AB_10597999 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P14621 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ACYP2, also named muscle-type acylphosphatase (ACYM), is a small cytoplasmic hydrolase (EC 3.6.1.7) encoded by the ACYP2 gene at human chromosome 2p16.2, with UniProt ID P14621. It catalyzes hydrolysis of acyl phosphate intermediates of ion pumps, especially sarcoplasmic reticulum Ca²⁺/Mg²⁺-ATPase, regulating intracellular calcium homeostasis and skeletal muscle differentiation. Widely expressed in muscle, brain and tumor tissues, ACYP2 exerts dual oncogenic or tumor-suppressive effects across malignancies. In glioma, it boosts Ca²⁺ efflux to activate c-Myc/STAT3 and drives temozolomide resistance; in hepatocellular carcinoma, it inhibits KCNN4/ERK and TERT to suppress tumor progression. It also participates in glycolysis, cell apoptosis and ion transport, serving as a potential prognostic biomarker for multiple cancers. (PMID: 32517717, 39267048)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ACYP2 antibody 12302-1-AP | Download protocol |
| WB protocol for ACYP2 antibody 12302-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
