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验证数据展示

  • WB analysis using 11316-1-AP

    WB analysis using 11316-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11316-1-AP (Aminoacylase 1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 11316-1-AP

    IHC staining of human prostate cancer using 11316-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11316-1-AP (Aminoacylase 1 antibody at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with ACY1 antibody included
  • IHC staining of human prostate cancer using 11316-1-AP

    IHC staining of human prostate cancer using 11316-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11316-1-AP (Aminoacylase 1 antibody at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with ACY1 antibody included
  • WB analysis of HepG2 using 11316-1-AP

    WB analysis of HepG2 using 11316-1-AP

    WB result of ACY1 antibody (11316-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACY1 transfected HepG2 cells.

  • KD/KO Validated

ACY1 Polyclonal antibody

ACY1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11316-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ACY 1, ACY-1, aminoacylase 1, Aminoacylase-1, EC:3.5.1.14

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, HepG2 cells, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ACY1 Ready-To-Use IHC Kit. ACY1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11316-1-AP targets ACY1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag1813

Product name: Recombinant human ACY1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 109-408 aa of BC014112

Sequence: RGAQDMKCVSIQYLEAVRRLKVEGHRFPRTIHMTFVPDEEVGGHQGMELFVQRPEFHALRAGFALDEGIANPTDAFTVFYSERSPWWVRVTSTGRPGHASRFMEDTAAEKLHKVVNSILAFREKEWQRLQSNPHLKEGSVTSVNLTKLEGGVAYNVIPATMSASFDFRVAPDVDFKAFEEQLQSWCQAAGEGVTLEFAQKWMHPQVTPTDDSNPWWAAFSRVCKDMNLTLEPEIMPAATDNRYIRAVGVPALGFSPMNRTPVLLHDHDERLHEAVFLRGVDIYTRLLPALASVPALPSDS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 aminoacylase 1
别名 ACY 1, ACY-1, aminoacylase 1, Aminoacylase-1, EC:3.5.1.14
计算分子量40.8 kDa
观测分子量 46 kDa
GenBank蛋白编号BC014112
基因名称 ACY1
Gene ID (NCBI) 95
RRIDAB_2877757
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ03154
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Aminoacylase 1 (ACY1) is a cytosolic enzyme that is widely distributed in mammalian tissues and catalyzes the hydrolysis of acylated amino acids into amino acids and acyl groups. The main functions of ACY1 are to accelerate the hydrolysis of N-acetylated peptides, especially N-acetylated neutral aliphatic amino acids, and participate in protein synthesis and turnover through the release of free amino acids.

实验方案

Product Specific Protocols
IHC protocol for ACY1 antibody 11316-1-APDownload protocol
WB protocol for ACY1 antibody 11316-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Heliyon

FYN-mediated phosphorylation of BCKDK at Y151 promotes GBM proliferation by increasing the oncogenic metabolite N-acetyl-L-alanine

Authors - Ling Zou
View Article
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