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验证数据展示

  • WB analysis using 11316-1-AP

    WB analysis using 11316-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11316-1-AP (Aminoacylase 1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 11316-1-AP

    IHC staining of human prostate cancer using 11316-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11316-1-AP (Aminoacylase 1 antibody at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with ACY1 antibody included
  • IHC staining of human prostate cancer using 11316-1-AP

    IHC staining of human prostate cancer using 11316-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11316-1-AP (Aminoacylase 1 antibody at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with ACY1 antibody included

ACY1 Polyclonal antibody

ACY1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11316-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ACY 1, ACY-1, aminoacylase 1, Aminoacylase-1, EC:3.5.1.14

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ACY1 Ready-To-Use IHC Kit. ACY1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11316-1-AP targets ACY1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag1813

Product name: Recombinant human ACY1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 109-408 aa of BC014112

Sequence: RGAQDMKCVSIQYLEAVRRLKVEGHRFPRTIHMTFVPDEEVGGHQGMELFVQRPEFHALRAGFALDEGIANPTDAFTVFYSERSPWWVRVTSTGRPGHASRFMEDTAAEKLHKVVNSILAFREKEWQRLQSNPHLKEGSVTSVNLTKLEGGVAYNVIPATMSASFDFRVAPDVDFKAFEEQLQSWCQAAGEGVTLEFAQKWMHPQVTPTDDSNPWWAAFSRVCKDMNLTLEPEIMPAATDNRYIRAVGVPALGFSPMNRTPVLLHDHDERLHEAVFLRGVDIYTRLLPALASVPALPSDS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 aminoacylase 1
别名 ACY 1, ACY-1, aminoacylase 1, Aminoacylase-1, EC:3.5.1.14
计算分子量40.8 kDa
观测分子量 46 kDa
GenBank蛋白编号BC014112
基因名称 ACY1
Gene ID (NCBI) 95
RRIDAB_2877757
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ03154
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ACY1 antibody 11316-1-APDownload protocol
IHC protocol for ACY1 antibody 11316-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Heliyon

FYN-mediated phosphorylation of BCKDK at Y151 promotes GBM proliferation by increasing the oncogenic metabolite N-acetyl-L-alanine

Authors - Ling Zou
View Article