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ACVR1B Recombinant antibody

ACVR1B Recombinant Antibody for FC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

FC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230260E12

Cat No : 83026-5-RR

Print datasheet

Synonyms

activin A receptor, type IB, Activin receptor like kinase 4, Activin receptor type 1B, Activin receptor type IB, ACTR IB, ACTRIB, ACVR1B, ACVRLK4, ALK 4, ALK4, SKR2

验证数据展示

  • IF Staining of NIH/3T3 using 83026-5-RR

    IF Staining of NIH/3T3 using 83026-5-RR

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using ACVR1B antibody (83026-5-RR, Clone: 230260E12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HEK-293 using 83026-5-RR

    FC experiment of HEK-293 using 83026-5-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Anti-Human ACVR1B (83026-5-RR, Clone:230260E12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inNIH/3T3 cells
Positive FC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83026-5-RR targets ACVR1B in IF, FC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications FC, IF/ICC, ELISA
Tested Reactivity Human, mouse
Immunogen ACVR1B fusion protein Ag0129 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name activin A receptor, type IB
Synonyms activin A receptor, type IB, Activin receptor like kinase 4, Activin receptor type 1B, Activin receptor type IB, ACTR IB, ACTRIB, ACVR1B, ACVRLK4, ALK 4, ALK4, SKR2
Calculated Molecular Weight 57 kDa
GenBank Accession NumberBC000254
Gene Symbol ACVR1B
Gene ID (NCBI) 91
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36896
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACVR1B (ALK4, activin A receptor type 1B) is a transforming growth factor-beta (TGF-beta) superfamily member (PMID: 37020544). A potential role for ACVR1B in regulating muscle mass is that it is part of the transforming growth factor b (TGFb) pathway regulating myostatin (PMID: 21063444).

实验方案

Product Specific Protocols
IF protocol for ACVR1B antibody 83026-5-RRDownload protocol
FC protocol for ACVR1B antibody 83026-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols