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验证数据展示

  • WB analysis using 17022-1-AP

    WB analysis using 17022-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17022-1-AP (ACTR3B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 17022-1-AP

    WB analysis of A431 using 17022-1-AP

    WB result of ACTR3B antibody (17022-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACTR3B transfected A431 cells.

  • IHC staining of human stomach cancer using 17022-1-AP

    IHC staining of human stomach cancer using 17022-1-AP

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 17022-1-AP (ACTR3B antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 17022-1-AP

    IHC staining of human stomach cancer using 17022-1-AP

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 17022-1-AP (ACTR3B antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

ACTR3B Polyclonal antibody

ACTR3B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17022-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Actin-related protein ARP4, Actin-related protein 3B, Actin-like protein 3B, Actin related protein ARP4, Actin related protein 3B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, mouse pancreas tissue, HepG2 cells, K-562 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17022-1-AP targets ACTR3B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10218

Product name: Recombinant human ACTR3B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 156-418 aa of BC008682

Sequence: RQVGERTLTGIVIDSGDGVTHVIPVAEGYVIGSCIKHIPIAGRDITYFIQQLLREREVGIPPEQSLETAKAIKEKYCYICPDIVKEFAKYDVDPRKWIKQYTGINAINQKKFVIDVGYERFLGPEIFFHPEFANPDFMESISDVVDEVIQNCPIDVRRPLYKNVVLSGGSTMFRDFGRRLQRDLKRVVDARLRLSEELSGGRIKPKPVEVQVVTHHMQRYAVWFGGSMLASTPEFFQVCHTKKDYEEYGPSICRHNPVFGVMS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ARP3 actin-related protein 3 homolog B (yeast)
别名 Actin-related protein ARP4, Actin-related protein 3B, Actin-like protein 3B, Actin related protein ARP4, Actin related protein 3B
计算分子量 418 aa, 48 kDa
观测分子量 48 kDa
GenBank蛋白编号BC008682
基因名称 ACTR3B
Gene ID (NCBI) 57180
RRIDAB_2305069
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P1U1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ACTR3B antibody 17022-1-APDownload protocol
IHC protocol for ACTR3B antibody 17022-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols