ARP3/ARP3B Monoclonal antibody

ARP3/ARP3B Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit, pig

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D12C7

Cat No : 68439-1-Ig

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Synonyms

Actin like protein 3, Actin related protein 3, ACTR3, ARP3



经过测试的应用

Positive WB detected inCalu-3 cells, A549 cells, U2OS cells, HeLa cells, Human peripheral blood platelets, Pig lung tissue, Rabbit lung tissue, Rat lung tissue, Mouse lung tissue
Positive IHC detected inmouse brain tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ARP3 Ready-To-Use IHC Kit. ARP3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68439-1-Ig targets ARP3/ARP3B in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, mouse, rat, rabbit, pig
Immunogen ARP3/ARP3B fusion protein Ag5017 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ARP3 actin-related protein 3 homolog (yeast)
Synonyms Actin like protein 3, Actin related protein 3, ACTR3, ARP3
Calculated Molecular Weight 418 aa 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC044590
Gene Symbol ACTR3
Gene ID (NCBI) 10096
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP61158
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARP3 (also known as ACTR3) is a major constituent of the ARP2/3 complex that is necessary for nucleating actin polymerization at filament branches. ARP3 localizes to the lamellipodia of stationary and locomoting fibroblasts. Overexpression of ARP3 has been reported in several types of cancer cells. This antibody recognizes endogenous ARP3 and may cross-react with ATCR3B.

实验方案

Product Specific Protocols
WB protocol for ARP3/ARP3B antibody 68439-1-IgDownload protocol
IHC protocol for ARP3/ARP3B antibody 68439-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols