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验证数据展示

  • WB analysis using 11023-1-AP

    WB analysis using 11023-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11023-1-AP (ACTR1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 11023-1-AP

    IHC staining of human breast cancer using 11023-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11023-1-AP (ACTR1B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 11023-1-AP

    IHC staining of human breast cancer using 11023-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11023-1-AP (ACTR1B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ACTR1A/ACTR1B Polyclonal antibody

ACTR1A/ACTR1B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11023-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

ACTR1B, Beta centractin, ARP1B, ACTR1A, Actin-related protein 1B

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse heart
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:300
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11023-1-AP targets ACTR1A/ACTR1B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag1467

Product name: Recombinant human ACTR1B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-376 aa of BC010090

Sequence: MESYDIIANQPVVIDNGSGVIKAGFAGDQIPKYCFPNYVGRPKHMRVMAGALEGDLFIGPKAEEHRGLLTIRYPMEHGVVRDWNDMERIWQYVYSKDQLQTFSEEHPVLLTEAPLNPSKNREKAAEVFFETFNVPALFISMQAVLSLYATGRTTGVVLDSGDGVTHAVPIYEGFAMPHSIMRVDIAGRDVSRYLRLLLRKEGVDFHTSAEFEVVRTIKERACYLSINPQKDEALETEKVQYTLPDGSTLDVGPARFRAPELLFQPDLVGDESEGLHEVVAFAIHKSDMDLRRTLFANIVLSGGSTLFKGFGDRLLSEVKKLAPKDIKIKISAPQERLYSTWIGGSILASLDTFKKMWVSKKEYEEDGSRAIHRKTF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ARP1 actin-related protein 1 homolog B, centractin beta (yeast)
别名 ACTR1B, Beta centractin, ARP1B, ACTR1A, Actin-related protein 1B
计算分子量 42 kDa
观测分子量 42-50 kDa
GenBank蛋白编号BC010090
基因名称 ACTR1B
Gene ID (NCBI) 10120
RRIDAB_2877742
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP42025
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACTR1B (also known as beta centractin) is a part of the 20S dynactin complex. It colocalizes with centrosomes and vesicular (membranous) structures and is involved in cell division. ACTR1B and ACTR1A share 90% amino acid identity, and this antibody recognizes both ACTR1B and ACTR1A.

实验方案

Product Specific Protocols
IHC protocol for ACTR1A/ACTR1B antibody 11023-1-APDownload protocol
WB protocol for ACTR1A/ACTR1B antibody 11023-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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