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验证数据展示

  • WB analysis using 66628-1-Ig (same clone as 66628-1-PBS)

    WB analysis using 66628-1-Ig (same clone as 66628-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66628-1-Ig (ACTN4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66628-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66628-1-Ig (same clone as 66628-1-PBS)

    IHC staining of human breast cancer using 66628-1-Ig (same clone as 66628-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66628-1-Ig (ACTN4 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66628-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

ACTN4 Monoclonal antibody, PBS Only

ACTN4 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66628-1-PBS

产品说明书

CloneNo. 1E12B8

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

WB, IHC, Indirect ELISA

别名

ACTININ 4, actinin, alpha 4, ACTN4, Alpha actinin 4, F actin cross linking protein, FSGS, FSGS1, Non muscle alpha actinin 4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66628-1-PBS targets ACTN4 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 ACTN4 fusion protein Ag13549 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 actinin, alpha 4
别名 ACTININ 4, actinin, alpha 4, ACTN4, Alpha actinin 4, F actin cross linking protein, FSGS, FSGS1, Non muscle alpha actinin 4
计算分子量 105 kDa
观测分子量 105 and 80 kDa
GenBank蛋白编号BC005033
基因名称 ACTN4
Gene ID (NCBI) 81
RRIDAB_2881988
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO43707
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACTN4 (alpha actinin 4) is a ubiquitous actin-binding protein that cross-links actin filaments into bundles to form filopodia, and has a crucial role in cytoskeletal organization. ACTN4 is colocalized with actin stress fibers and is dispersed in the cytoplasm and in the nucleus. Increased levels of ACTN4 have been associated with cancer metastasis, indicating its implication in cancer cell migration and metastasis. Defects in ACTN4 cause glomerular disease. This antibody recognizes 105 and 80 kDa of endogenous ACTN4 protein in various lysates. (22370640, 10700177)