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验证数据展示

  • IF Staining of Human iPSC derived cardiomyocyte using 14221-1-AP

    IF Staining of Human iPSC derived cardiomyocyte using 14221-1-AP

    Human iPSC derived cardiomyocyte in 2D culture, with alpha actinin (14221-1-AP) and DAPI. Image courtesy of Chandan Kadur Nagaraju, Experimental Cardiology, KU Leuven, Belgium.

  • IF Staining of mouse heart using 14221-1-AP

    IF Staining of mouse heart using 14221-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 14221-1-AP (ACTN2 antibody) at dilution of 1:400 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse heart using 14221-1-AP

    IHC staining of mouse heart using 14221-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14221-1-AP (ACTN2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 14221-1-AP

    WB analysis using 14221-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14221-1-AP (ACTN2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 14221-1-AP

    IP experiment of HeLa using 14221-1-AP

    IP result of anti-ACTN2 (IP:14221-1-AP, 4ug; Detection:14221-1-AP 1:1000) with HeLa cells lysate 1080ug.

  • IHC staining of mouse heart using 14221-1-AP

    IHC staining of mouse heart using 14221-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14221-1-AP (ACTN2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 14221-1-AP

    IHC staining of mouse skeletal muscle using 14221-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14221-1-AP (ACTN2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 14221-1-AP

    IHC staining of mouse skeletal muscle using 14221-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14221-1-AP (ACTN2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse heart using 14221-1-AP

    IF Staining of mouse heart using 14221-1-AP

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse heart tissue using 14221-1-AP (ACTN2 antibody) at dilution of 1:400.

  • IF Staining of C2C12 using 14221-1-AP

    IF Staining of C2C12 using 14221-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using ACTN2 antibody (14221-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 594phalloidin (red).

ACTN2 Polyclonal antibody

ACTN2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, IP, ELISA
Cat No. 14221-1-AP

产品说明书

引用文献(42)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (2)

应用

WB, IHC, IF/ICC, IF-P, IF-Fro, IP, ELISA and More (1)

别名

Actinin alpha 2, actinin, alpha 2, ACTN 2, Alpha actinin 2, Alpha-actinin skeletal muscle isoform 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, C2C12 cells, mouse lung tissue, mouse skeletal muscle tissue, mouse heart, mouse kidney, rat skeletal muscle
Positive IP detected inHeLa cells
Positive IHC detected inmouse heart tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue
Positive IF-Fro detected inmouse heart tissue
Positive IF/ICC detected inC2C12 cells, NIH/3T3 cells, Human iPSC derived cardiomyocyte
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)-FROIF-FRO : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14221-1-AP targets ACTN2 in WB, IHC, IF/ICC, IF-P, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IF-Fro, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, canine, chicken
免疫原

CatNo: Ag5459

Product name: Recombinant human ACTN2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-301 aa of BC051770

Sequence: MNQIEPGVQYNYVYDEDEYMIQEEEWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRNGLKLMLLLEVISGERLPKPDRGKMRFHKIANVNKALDYIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYRNVNIQNFHTSWKDGLGLCALIHRHRPDLIDYSKLNKDDPIGNINLAMEIAEKHLDIPKMLDAEDIVNTPKPDERAIMTYVSCFYHAFAGAEQAETAANRICKVLAVNQENERLMEEYERLASELLEWIRRTI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 actinin, alpha 2
别名 Actinin alpha 2, actinin, alpha 2, ACTN 2, Alpha actinin 2, Alpha-actinin skeletal muscle isoform 2
计算分子量 104 kDa
观测分子量 103 kDa
GenBank蛋白编号BC051770
基因名称 ACTN2
Gene ID (NCBI) 88
RRIDAB_2221547
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP35609
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Alpha actinin 2 (ACTN2) belongs to the alpha-actinin family and is expressed in both skeletal and cardiac muscles and functions to anchor myofibrillar actin thin filaments and titin to Z-discs (PMID: 30701273). ACTN2 is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. Mutations in ACTN2 are associated with hypertrophic cardiomyopathy, as well as dilated cardiomyopathy and endocardial fibroelastosis (PMID: 20022194, 14567970).

实验方案

Product Specific Protocols
IF protocol for ACTN2 antibody 14221-1-APDownload protocol
IHC protocol for ACTN2 antibody 14221-1-APDownload protocol
IP protocol for ACTN2 antibody 14221-1-APDownload protocol
WB protocol for ACTN2 antibody 14221-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP,IF

Signal Transduct Target Ther

Targeting intracellular cholesterol imbalance rescues sarcomere-ER contact site signaling and ER remodeling in dilated cardiomyopathy.

Authors - Nadezda Ignatyeva
View Article
rat

Adv Mater

Exoskeleton Partial-Coated Stem Cells for Infarcted Myocardium Restoring

Authors - Huihui He
View Article
mouseWB,IF

Nat Commun

Microskeletal stiffness promotes aortic aneurysm by sustaining pathological vascular smooth muscle cell mechanosensation via Piezo1.

Authors - Weiyi Qian
View Article
humanIF

Nat Commun

Essential role of NONO-HOXA1-Wnt axis in cardiomyocyte differentiation.

Authors - Zhiyu Feng
View Article
rat,mouseWB

Acta Pharm Sin B

Parkin inhibits iron overload-induced cardiomyocyte ferroptosis by ubiquitinating ACSL4 and modulating PUFA-phospholipids metabolism

Authors - Dandan Xiao
View Article
humanWB

Cell Rep Med

Phase 1/2 trial of brogidirsen: Dual-targeting antisense oligonucleotides for exon 44 skipping in Duchenne muscular dystrophy

Authors - Hirofumi Komaki
View Article
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