验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, rat testis tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17355-1-AP targets ACTL7A in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag10613 Product name: Recombinant human ACTL7A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-280 aa of BC014610 Sequence: MWAPPAAIMGDGPTKKVGNQAPLQTQALQTASLRDGPAKRAVWVRHTSSEPQEPTESKAAKERPKQEVTKAVVVDLGTGYCKCGFAGLPRPTHKISTTVGKPYMETAKTGDNRKETFVGQELNNTNVHLKLVNPLRHGIIVDWDTVQDIWEYLFRQEMKIAPEEHAVLVSDPPLSPHTNREKYAEMLFEAFNTPAMHIAYQSRLSMYSYGRTSGLVVEVGHGVSYVVPIYEGYPLPSITGRLDYAGSDLTAYLLGLLNSAGNEFTQDQMGIVEDIKKKCC 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | actin-like 7A |
| 别名 | Actin like 7 alpha, actin like 7A, Actin like protein 7A, ACTL7A |
| 计算分子量 | 435 aa, 49 kDa |
| 观测分子量 | 49 kDa |
| GenBank蛋白编号 | BC014610 |
| 基因名称 | ACTL7A |
| Gene ID (NCBI) | 10881 |
| RRID | AB_2273677 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y615 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ACTL7a is located in the acrosome and tail of mature spermatozoa and plays an important role in spermiogenesis. Recently mutation of ACTL7A has been linked to infertility.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ACTL7A antibody 17355-1-AP | Download protocol |
| WB protocol for ACTL7A antibody 17355-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Am J Hum Genet Homozygous pathogenic variants in ACTL9 cause fertilization failure and male infertility in humans and mice. | ||
Hum Reprod ACROSIN deficiency causes total fertilization failure in humans by preventing the sperm from penetrating the zona pellucida | ||
Hum Reprod Deletion of ACTRT1 is associated with male infertility as sperm acrosomal ultrastructural defects and fertilization failure in human | ||
Hum Reprod Loss of SPACA1 function causes autosomal recessive globozoospermia by damaging the acrosome-acroplaxome complex. | ||
Hum Reprod Novel bi-allelic variants in ACTL7A are associated with male infertility and total fertilization failure. | ||
Development Loss of perinuclear theca ACTRT1 causes acrosome detachment and severe male subfertility in mice. |
