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验证数据展示

  • WB analysis using 31849-1-AP (same clone as 31849-1-PBS)

    WB analysis using 31849-1-AP (same clone as 31849-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31849-1-AP (ACTG2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31849-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse stomach using 31849-1-AP (same clone as 31849-1-PBS)

    IHC staining of mouse stomach using 31849-1-AP (same clone as 31849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 31849-1-AP (ACTG2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31849-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse stomach using 31849-1-AP (same clone as 31849-1-PBS)

    IHC staining of mouse stomach using 31849-1-AP (same clone as 31849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 31849-1-AP (ACTG2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31849-1-PBS in a different storage buffer formulation.

ACTG2 Polyclonal antibody, PBS Only

ACTG2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 31849-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

ACTA3, Actin, gamma-enteric smooth muscle, Actin, gamma-enteric smooth muscle, intermediate form, ACTL3, ACTSG

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

31849-1-PBS targets ACTG2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag36024

Product name: Recombinant human ACTG2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-100 aa of BC094877

Sequence: MCEEETTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 actin, gamma 2, smooth muscle, enteric
别名 ACTA3, Actin, gamma-enteric smooth muscle, Actin, gamma-enteric smooth muscle, intermediate form, ACTL3, ACTSG
计算分子量42 kDa
观测分子量42 kDa
GenBank蛋白编号BC094877
基因名称 ACTG2
Gene ID (NCBI) 72
RRIDAB_3670128
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP63267
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Actin gamma 2 (ACTG2, also known as ACT, ACTE, VSCM, ACTA3, ACTL3, and ACTSG) is a highly conserved protein involved in various types of cell motility and ubiquitously expressed in all eukaryotic cells. ACTG2 mutations would cause congenital distended bladder, microcolon, and intestinal hypoperistalsis (PMID: 24337657). It also has been reported that ACTG2 promotes hepatocellular carcinoma (HCC) migration and invasion through the Notch1 signaling pathway, which might serve as a new intervention target for the treatment of HCC (PMID: 28385530).

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