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验证数据展示

  • WB analysis using 84444-1-RR

    WB analysis using 84444-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84444-1-RR (ACSM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 84444-1-RR

    IHC staining of mouse liver using 84444-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84444-1-RR (ACSM5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HepG2 using 84444-1-RR

    FC experiment of HepG2 using 84444-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ACSM5 Recombinant antibody (84444-1-RR, Clone:241400E7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84444-1-RR

    Affinity and Kinetic Characterization of 84444-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84444-1-RR against Human ACSM5 were performed. The affinity constant is below 1 pM.

ACSM5 Recombinant antibody

ACSM5 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 84444-1-RR

产品说明书

CloneNo. 241400E7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), ELISA

别名

241400E7, ACSM 5, Acyl-CoA synthetase medium-chain family member 5, Acyl-coenzyme A synthetase ACSM5, mitochondrial, EC:6.2.1.2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue, human kidney tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84444-1-RR targets ACSM5 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ACSM5 fusion protein Ag9373 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 acyl-CoA synthetase medium-chain family member 5
别名 241400E7, ACSM 5, Acyl-CoA synthetase medium-chain family member 5, Acyl-coenzyme A synthetase ACSM5, mitochondrial, EC:6.2.1.2
计算分子量 208 aa, 23 kDa
观测分子量 60-65 kDa
GenBank蛋白编号BC016703
基因名称 ACSM5
Gene ID (NCBI) 54988
RRIDAB_3671969
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ6NUN0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACSM5(Acyl-CoA synthetase medium-chain family member 5) is also named as MACS3 and belongs to the ATP-dependent AMP-binding enzyme family. ACSM5 has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). This protein has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for ACSM5 antibody 84444-1-RRDownload protocol
IHC protocol for ACSM5 antibody 84444-1-RRDownload protocol
FC protocol for ACSM5 antibody 84444-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols