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验证数据展示

  • WB analysis using 84444-1-RR (same clone as 84444-1-PBS)

    WB analysis using 84444-1-RR (same clone as 84444-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84444-1-RR (ACSM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84444-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 84444-1-RR (same clone as 84444-1-PBS)

    IHC staining of mouse liver using 84444-1-RR (same clone as 84444-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84444-1-RR (ACSM5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84444-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 84444-1-RR (same clone as 84444-1-PBS)

    FC experiment of HepG2 using 84444-1-RR (same clone as 84444-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ACSM5 Recombinant antibody (84444-1-RR, Clone:241400E7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84444-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84444-1-RR

    Affinity and Kinetic Characterization of 84444-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84444-1-RR against Human ACSM5 were performed. The affinity constant is below 1 pM.

ACSM5 Recombinant monoclonal antibody, PBS Only

ACSM5 Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 84444-1-PBS

产品说明书

CloneNo. 241400E7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

241400E7, ACSM 5, Acyl-CoA synthetase medium-chain family member 5, Acyl-coenzyme A synthetase ACSM5, mitochondrial, EC:6.2.1.2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84444-1-PBS targets ACSM5 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9373

Product name: Recombinant human ACSM5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-208 aa of BC016703

Sequence: MRPWLRHLVLQALRNSRAFCGSHGKPAPLPVPQKIVATWEAISLGRQLVPEYFNFAHDVLDVWSRLEEAGHRPPNPAFWWVNGTGAEIKWSFEELGKQSRKAANVLGGACGLQPGDRMMLVLPRLPEWWLVSVACMRTGTVVIPGVTQLTEKDLKYRLQASRAKSIITSDSLAPRVDAISAECPSLQTKLLVSDSSRPGWLNFRELLR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 acyl-CoA synthetase medium-chain family member 5
别名 241400E7, ACSM 5, Acyl-CoA synthetase medium-chain family member 5, Acyl-coenzyme A synthetase ACSM5, mitochondrial, EC:6.2.1.2
计算分子量 208 aa, 23 kDa
观测分子量 60-65 kDa
GenBank蛋白编号BC016703
基因名称 ACSM5
Gene ID (NCBI) 54988
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ6NUN0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACSM5(Acyl-CoA synthetase medium-chain family member 5) is also named as MACS3 and belongs to the ATP-dependent AMP-binding enzyme family. ACSM5 has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). This protein has 2 isoforms produced by alternative splicing.

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