验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue | 
| Positive IHC detected in | human liver cancer tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 | 
推荐稀释比
| 应用 | 推荐稀释比 | 
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 | 
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below | 
产品信息
16591-1-AP targets ACSM5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description | 
| 文献引用应用 | WB | 
| 经测试反应性 | human, mouse, rat | 
| 文献引用反应性 | mouse | 
| 免疫原 | CatNo: Ag9373 Product name: Recombinant human ACSM5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-208 aa of BC016703 Sequence: MRPWLRHLVLQALRNSRAFCGSHGKPAPLPVPQKIVATWEAISLGRQLVPEYFNFAHDVLDVWSRLEEAGHRPPNPAFWWVNGTGAEIKWSFEELGKQSRKAANVLGGACGLQPGDRMMLVLPRLPEWWLVSVACMRTGTVVIPGVTQLTEKDLKYRLQASRAKSIITSDSLAPRVDAISAECPSLQTKLLVSDSSRPGWLNFRELLR种属同源性预测 | 
| 宿主/亚型 | Rabbit / IgG | 
| 抗体类别 | Polyclonal | 
| 产品类型 | Antibody | 
| 全称 | acyl-CoA synthetase medium-chain family member 5 | 
| 别名 | MACS3, EC:6.2.1.2, Acyl-coenzyme A synthetase ACSM5, mitochondrial, Acyl-CoA synthetase medium-chain family member 5, ACSM 5 | 
| 计算分子量 | 208 aa, 23 kDa | 
| 观测分子量 | 65 kDa | 
| GenBank蛋白编号 | BC016703 | 
| 基因名称 | ACSM5 | 
| Gene ID (NCBI) | 54988 | 
| RRID | AB_2878284 | 
| 偶联类型 | Unconjugated | 
| 形式 | Liquid | 
| 纯化方式 | Antigen affinity purification | 
| UNIPROT ID | Q6NUN0 | 
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. | 
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. | 
背景介绍
ACSM5(Acyl-CoA synthetase medium-chain family member 5) is also named as MACS3 and belongs to the ATP-dependent AMP-binding enzyme family. ACSM5 has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). This protein has 2 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ACSM5 antibody 16591-1-AP | Download protocol | 
| WB protocol for ACSM5 antibody 16591-1-AP | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 








