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验证数据展示

  • IHC staining of human liver cancer using 16591-1-AP

    IHC staining of human liver cancer using 16591-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16591-1-AP (ACSM5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 16591-1-AP

    IHC staining of human liver cancer using 16591-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16591-1-AP (ACSM5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 16591-1-AP

    WB analysis using 16591-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16591-1-AP (ACSM5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 16591-1-AP

    IHC staining of mouse liver using 16591-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16591-1-AP (ACSM5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ACSM5 Polyclonal antibody

ACSM5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16591-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

MACS3, EC:6.2.1.2, Acyl-coenzyme A synthetase ACSM5, mitochondrial, Acyl-CoA synthetase medium-chain family member 5, ACSM 5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16591-1-AP targets ACSM5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 ACSM5 fusion protein Ag9373 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 acyl-CoA synthetase medium-chain family member 5
别名 MACS3, EC:6.2.1.2, Acyl-coenzyme A synthetase ACSM5, mitochondrial, Acyl-CoA synthetase medium-chain family member 5, ACSM 5
计算分子量 208 aa, 23 kDa
观测分子量 65 kDa
GenBank蛋白编号BC016703
基因名称 ACSM5
Gene ID (NCBI) 54988
RRIDAB_2878284
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6NUN0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACSM5(Acyl-CoA synthetase medium-chain family member 5) is also named as MACS3 and belongs to the ATP-dependent AMP-binding enzyme family. ACSM5 has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). This protein has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for ACSM5 antibody 16591-1-APDownload protocol
IHC protocol for ACSM5 antibody 16591-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Metab

Calponin 2 harnesses metabolic reprogramming to determine kidney fibrosis

Authors - Yuan Gui
View Article