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验证数据展示

  • WB analysis using 13847-1-AP

    WB analysis using 13847-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13847-1-AP (ACSL6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 13847-1-AP

    IP experiment of mouse brain using 13847-1-AP

    IP result of anti-ACSL6 (IP:13847-1-AP, 4ug; Detection:13847-1-AP 1:2000) with mouse brain tissue lysate 2120 ug.

  • IHC staining of human urothelial carcinoma using 13847-1-AP

    IHC staining of human urothelial carcinoma using 13847-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 13847-1-AP (ACSL6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 13847-1-AP

    IHC staining of human pancreas cancer using 13847-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13847-1-AP (ACSL6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ACSL6 Polyclonal antibody

ACSL6 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 13847-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

ACSL 6, Arachidonate--CoA ligase, EC:6.2.1.15, EC:6.2.1.3, FACL6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman pancreas cancer tissue, human urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13847-1-AP targets ACSL6 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ACSL6 fusion protein Ag4817 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 acyl-CoA synthetase long-chain family member 6
别名 ACSL 6, Arachidonate--CoA ligase, EC:6.2.1.15, EC:6.2.1.3, FACL6
计算分子量 78 kDa
观测分子量74 kDa
GenBank蛋白编号BC047453
基因名称 ACSL6
Gene ID (NCBI) 23305
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UKU0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACSL6 (Acyl-CoA Synthetase Long Chain Family Member 6) is a long-chain lipoyl-coenzyme A synthetase that is mainly responsible for catalyzing the binding of long-chain fatty acids to coenzyme A to form acyl-coenzyme A (Acyl-CoA). ACSL6 is involved in fatty acid metabolic transporter and the synthesis of cell membrane phospholipids, which affects the cell structure and function. ACSL6 plays an extremely important role in the pathogenesis of cancers such as hepatocellular carcinoma, lung cancer and neuroblastoma, suggesting that it may serve as a biomarker and therapeutic target for cancer therapy.

实验方案

Product Specific Protocols
WB protocol for ACSL6 antibody 13847-1-APDownload protocol
IHC protocol for ACSL6 antibody 13847-1-APDownload protocol
IP protocol for ACSL6 antibody 13847-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols