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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis using 66617-1-Ig (same clone as 66617-1-PBS)

    WB analysis using 66617-1-Ig (same clone as 66617-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

  • IHC staining of human cerebellum using 66617-1-Ig (same clone as 66617-1-PBS)

    IHC staining of human cerebellum using 66617-1-Ig (same clone as 66617-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66617-1-Ig (ACSL4 antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACSL4 antibody included
  • IHC staining of human cerebellum using 66617-1-Ig (same clone as 66617-1-PBS)

    IHC staining of human cerebellum using 66617-1-Ig (same clone as 66617-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66617-1-Ig (ACSL4 antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACSL4 antibody included
  • WB analysis of HepG2 using 66617-1-Ig (same clone as 66617-1-PBS)

    WB analysis of HepG2 using 66617-1-Ig (same clone as 66617-1-PBS)

    WB result of ACSL4 antibody (66617-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSL4 transfected HepG2 cells. This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293T using 66617-1-Ig (same clone as 66617-1-PBS)

    FC experiment of HEK-293T using 66617-1-Ig (same clone as 66617-1-PBS)

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human ACSL4 (66617-1-Ig, Clone:1H5D3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66617-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

ACSL4/FACL4 Monoclonal antibody, PBS Only

ACSL4/FACL4 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 66617-1-PBS

产品说明书

CloneNo. 1H5D3

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

ACSL4, FACL4, 1H5D3, ACS4, ACSL 4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ACSL4 Ready-To-Use IHC Kit. ACSL4 primary antibody included.

产品信息

66617-1-PBS targets ACSL4/FACL4 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag18085

Product name: Recombinant human ACSL4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 143-236 aa of BC034959

Sequence: LGKEAVVHGLNESEASYLITSVELLESKLKTALLDISCVKHIIYVDNKAINKAEYPEGFEIHSMQSVEELGSNPENLGIPPSRPTPSDMAIVMY

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 acyl-CoA synthetase long-chain family member 4
别名 ACSL4, FACL4, 1H5D3, ACS4, ACSL 4
计算分子量 711 aa, 79 kDa
观测分子量 74 kDa
GenBank蛋白编号BC034959
基因名称 ACSL4
Gene ID (NCBI) 2182
RRIDAB_2881977
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO60488
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Acyl-CoA synthetase long-chain family 4 (ACSL4) is a key enzyme that catalyzes long-chain fatty acids activation, and abnormal expression of ACSL4 is closely related to various biological responses, including steroidogenesis, inflammation response, cell death, immune activation response, and so on. Specifically, ACSL4 participates in ferroptosis, a promising target for tumor therapeutics.