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验证数据展示

  • IHC staining of mouse colon using 84033-2-RR

    IHC staining of mouse colon using 84033-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84033-2-RR (ACSF2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 84033-2-RR

    IF Staining of A549 using 84033-2-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ACSF2 antibody (84033-2-RR, Clone: 241208C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of A549 using 84033-2-RR

    IF Staining of A549 using 84033-2-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ACSF2 antibody (84033-2-RR, Clone: 241208C3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84033-2-RR

    Affinity and Kinetic Characterization of 84033-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84033-2-RR against Human ACSF2 were performed. The affinity constant is 0.595 nM.

ACSF2 Recombinant monoclonal antibody

ACSF2 Uni-rAb® Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 84033-2-RR

产品说明书

CloneNo. 241208C3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA

别名

UNQ493/PRO1009, Medium-chain acyl-CoA ligase ACSF2, mitochondrial, EC:6.2.1.2, ACSF 2, 241208C3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84033-2-RR targets ACSF2 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag9199

Product name: Recombinant human ACSF2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 336-615 aa of BC012053

Sequence: FNGKKALEAISRERGTFLYGTPTMFVDILNQPDFSSYDISTMCGGVIAGSPAPPELIRAIINKINMKDLVVAYGTTENSPVTFAHFPEDTVEQKAESVGRIMPHTEARIMNMEAGTLAKLNTPGELCIRGYCVMLGYWGEPQKTEEAVDQDKWYWTGDVATMNEQGFCKIVGRSKDMIIRGGENIYPAELEDFFHTHPKVQEVQVVGVKDDRMGEEICACIRLKDGEETTVEEIKAFCKGKISHFKIPKYIVFVTNYPLTISGKIQKFKLREQMERHLNL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 acyl-CoA synthetase family member 2
别名 UNQ493/PRO1009, Medium-chain acyl-CoA ligase ACSF2, mitochondrial, EC:6.2.1.2, ACSF 2, 241208C3
计算分子量 615 aa, 68 kDa
GenBank蛋白编号BC012053
基因名称 ACSF2
Gene ID (NCBI) 80221
RRIDAB_3671600
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96CM8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for ACSF2 antibody 84033-2-RRDownload protocol
IHC protocol for ACSF2 antibody 84033-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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