验证数据展示
经过测试的应用
| Positive WB detected in | mouse lung tissue |
| Positive IHC detected in | human colon cancer tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16140-1-AP targets ACSF2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag9199 Product name: Recombinant human ACSF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 336-615 aa of BC012053 Sequence: FNGKKALEAISRERGTFLYGTPTMFVDILNQPDFSSYDISTMCGGVIAGSPAPPELIRAIINKINMKDLVVAYGTTENSPVTFAHFPEDTVEQKAESVGRIMPHTEARIMNMEAGTLAKLNTPGELCIRGYCVMLGYWGEPQKTEEAVDQDKWYWTGDVATMNEQGFCKIVGRSKDMIIRGGENIYPAELEDFFHTHPKVQEVQVVGVKDDRMGEEICACIRLKDGEETTVEEIKAFCKGKISHFKIPKYIVFVTNYPLTISGKIQKFKLREQMERHLNL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | acyl-CoA synthetase family member 2 |
| 别名 | UNQ493/PRO1009, Medium-chain acyl-CoA ligase ACSF2, mitochondrial, EC:6.2.1.2, ACSF 2 |
| 计算分子量 | 615 aa, 68 kDa |
| 观测分子量 | 55-70 kDa |
| GenBank蛋白编号 | BC012053 |
| 基因名称 | ACSF2 |
| Gene ID (NCBI) | 80221 |
| RRID | AB_2878222 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96CM8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ACSF2 (encoded by acyl-CoA synthetase family member 2) belongs to the acyl-CoA synthetase (ACS) family, activating fatty acids by forming a thioester bond with CoA.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ACSF2 antibody 16140-1-AP | Download protocol |
| IHC protocol for ACSF2 antibody 16140-1-AP | Download protocol |
| WB protocol for ACSF2 antibody 16140-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Prostaglandins Other Lipid Mediat Targeted Lipidomics Analysis of Lysine 179 Acetylation of ACSF2 in Rat Hepatic Stellate Cells | ||
Free Radic Biol Med Inhibition of ACSF2 protects against renal ischemia/reperfusion injury via mediating mitophagy in proximal tubular cells
| ||
Clin Cancer Res Biomarker identification and risk prediction model development for differentiated thyroid carcinoma lung metastasis based on primary lesion proteomics | ||
Mol Carcinog M2 macrophage exosome-derived Apoc1 promotes ferroptosis resistance in osteosarcoma by inhibiting ACSF2 deubiquitination |
