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验证数据展示

  • WB analysis using 12487-1-AP

    WB analysis using 12487-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 12487-1-AP (ACRBP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 12487-1-AP

    IHC staining of mouse testis using 12487-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12487-1-AP (ACRBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ACRBP Polyclonal antibody

ACRBP Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12487-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Acrosin-binding protein, Acrosin-binding protein, 60 kDa form, Acrosin-binding protein, mature form, Cancer/testis antigen 23, Cancer/testis antigen OY-TES-1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12487-1-AP targets ACRBP in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3180

Product name: Recombinant human ACRBP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 244-543 aa of BC033010

Sequence: AVSQLQTDSEPKFHSESLSSNPSSFAPRVREVESTPMIMENIQELIRSAQEIDEMNEIYDENSYWRNQNPGSLLQLPHTEALLVLCYSIVENTCIITPTAKAWKYMEEEILGFGKSVCDSLGRRHMSTCALCDFCSLKLEQCHSEASLQRQQCDTSHKTPFVSPLLASQSLSIGNQVGSPESGRFYGLDLYGGLHMDFWCARLATKGCEDVRVSGWLQTEFLSFQDGDFPTKICDTDYIQYPNYCSFKSQQCLMRNRNRKVSRMRCLQNETYSALSPGKSEDVVLRWSQEFSTLTLGQFG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 acrosin binding protein
别名 Acrosin-binding protein, Acrosin-binding protein, 60 kDa form, Acrosin-binding protein, mature form, Cancer/testis antigen 23, Cancer/testis antigen OY-TES-1
计算分子量 543 aa, 61 kDa
观测分子量60 kDa
GenBank蛋白编号BC033010
基因名称 ACRBP
Gene ID (NCBI) 84519
RRIDAB_3669141
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NEB7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ACRBP antibody 12487-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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