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验证数据展示

  • WB analysis using 87842-1-RR

    WB analysis using 87842-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87842-1-RR (ACPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate using 87842-1-RR

    IHC staining of human prostate using 87842-1-RR

    Immunohistochemical analysis of paraffin-embedded human prostate tissue slide using 87842-1-RR (PPAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PAP/ACPP antibody included
  • IHC staining of human prostate using 87842-1-RR

    IHC staining of human prostate using 87842-1-RR

    Immunohistochemical analysis of paraffin-embedded human prostate tissue slide using 87842-1-RR (PPAP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PAP/ACPP antibody included

ACPP Recombinant monoclonal antibody

ACPP Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 87842-1-RR

产品说明书

CloneNo. 253501A5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

PAP, 5'-NT, 5'-nucleotidase, Acid phosphatase 3, acid phosphatase, prostate

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inVCaP cells
Positive IHC detected inhuman prostate tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PAP/ACPP Ready-To-Use IHC Kit. PAP/ACPP primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87842-1-RR targets ACPP in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg6542

Product name: Recombinant human PPAP protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 33-386 aa of NM_001099.5

Sequence: KELKFVTLVFRHGDRSPIDTFPTDPIKESSWPQGFGQLTQLGMEQHYELGEYIRKRYRKFLNESYKHEQVYIRSTDVDRTLMSAMTNLAALFPPEGVSIWNPILLWQPIPVHTVPLSEDQLLYLPFRNCPRFQELESETLKSEEFQKRLHPYKDFIATLGKLSGLHGQDLFGIWSKVYDPLYCESVHNFTLPSWATEDTMTKLRELSELSLLSLYGIHKQKEKSRLQGGVLVNEILNHMKRATQIPSYKKLIMYSAHDTTVSGLQMALDVYNGLLPPYASCHLTELYFEKGEYFVEMYYRNETQHEPYPLMLPGCSPSCPLERFAELVGPVIPQDWSTECMTTNSHQGTEDSTD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 acid phosphatase, prostate
别名 PAP, 5'-NT, 5'-nucleotidase, Acid phosphatase 3, acid phosphatase, prostate
计算分子量45 kDa
观测分子量40-50 kDa
GenBank蛋白编号NM_001099.5
基因名称 ACPP
Gene ID (NCBI) 55
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP15309
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACPP is also named as PAP(prostatic acid phosphatase),5'-nucleotidase,thiamine monophosphatas and Belongs to the histidine acid phosphatase family.This enzyme is a homodimeric glycoprotein with a subunit molecular mass of 50 kDa and is an androgen-stimulated secretory product of the human prostate gland(PMID:8132635).It is also a non-specific phosphatase and has been implicated in the progression of prostate cancer, which inactivates LPA in human seminal plasma(PMID:15280042).

实验方案

Product Specific Protocols
IHC protocol for ACPP antibody 87842-1-RRDownload protocol
WB protocol for ACPP antibody 87842-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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