验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, MCF-7 cells, SW480 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27898-1-AP targets ACP5 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag27383 Product name: Recombinant human ACP5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 43-199 aa of BC025414 Sequence: HTAREMANAKEIARTVQILGADFILSLGDNFYFTGVQDINDKRFQETFEDVFSDRSLRKVPWYVLAGNHDHLGNVSAQIAYSKISKRWNFPSPFYRLHFKIPQTNVSVAIFMLDTVTLCGNSDDFLSQQPERPRDVKLARTQLSWLKKQLAAAREDY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | acid phosphatase 5, tartrate resistant |
| 别名 | ACP 5, EC:3.1.3.2, Tartrate-resistant acid ATPase, Tartrate-resistant acid phosphatase type 5, TR-AP |
| 计算分子量 | 325 aa, 37 kDa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | BC025414 |
| 基因名称 | ACP5 |
| Gene ID (NCBI) | 54 |
| RRID | AB_3742265 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P13686 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Tartrate-resistant acid phosphatase (TRAP/ACP5) is a metalloproteinase enzyme that maps to chromosome 19p13.2 and spans approximately 3 kb, belonging to the acid phosphatase family that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. Accumulating studies have demonstrated that ACP5 broadly participates in various malignant processes including uncontrolled proliferation, chemotherapy resistance, cellular invasion and metastasis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ACP5 antibody 27898-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
